钟状期小鼠上颌第一磨牙的拔除与原位移植模型  

Extraction and orthotopic transplantation model of the upper first molar at bell stage in mice

作　　者：李星瀚 田卫东[2] LI Xinghan;TIAN Weidong(Department of Stomatology, Shenzhen University General Hospital, Shenzhen 518000, China;State Key Laboratory of Oral Diseases, National Engineering Laboratory for Oral Regenerative Medicine, Department of Traumatic and Plastic Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China)

机构地区：[1]深圳大学总医院口腔科,广东深圳518000 [2]口腔疾病研究国家重点实验室,四川大学华西口腔医院口腔再生医学技术国家地方联合工程实验室,四川大学华西口腔医院创伤与整形外科,四川成都610041

出　　处：《口腔生物医学》2020年第2期76-80,共5页Oral Biomedicine

基　　金：国家重点研发计划(2017YFA01048000);深圳大学总医院人才助推计划(SUGH2018QD058)。

摘　　要：目的:为研究牙再生、牙根发育和牙萌出建立能模拟天然牙发育环境的动物模型。方法:选取钟状期小鼠上颌第一磨牙作为牙拔除与牙移植的研究对象,进行动物建模,并通过术后影像学和组织学观察与肾被膜下移植模型进行对比。结果:出生后第8天的小鼠上颌第一磨牙牙胚处于牙冠基本形成而牙根即将开始发育的阶段,适合用于本模型。相较肾被膜下移植模型,本模型移植牙胚的牙根发育更接近天然牙发育。结论:钟状期小鼠上颌第一磨牙牙胚拔除及移植模型是观察牙根发育和牙萌出的良好选择。Objective:Establishing an animal model which can simulate the natural tooth developing environment in physiological condition for the researches of tooth regeneration,root development and tooth eruption.Methods:The upper first molar of mouse at bell stage was used in this model for extraction and transplantation,comparing with the subrenal capsule transplantation model by radiological and histological observation postoperatively.Results:The tooth germ of upper first molar by the postnatal 8th day has almost formed the crown and the root development was about to begin,which was a suitable subject of study in this model.Comparing with that in the subrenal capsule transplantation model,the root development of transplanted tooth germ in this model showed more similar to that of the natural tooth.Conclusions:The extraction and orthotopic transplantation model of the upper first molar at bell stage in mice is a desired option for the observation of root development and tooth eruption.

关 键 词：牙胚 动物模型 牙再生 牙发育 

分 类 号：R782.12[医药卫生—口腔医学]