微小RNA-622对非小细胞肺癌A549细胞增殖、凋亡的影响  

Effects of microRNA-622 on the proliferation and apoptosis of non-small cell lung cancer A549 cells

作　　者：周海荣[1] 毕明宏[1] Zhou Hairong;Bi Minghong(Department of Oncology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,China)

机构地区：[1]蚌埠医学院第一附属医院肿瘤内科,安徽省蚌埠市233004

出　　处：《中华解剖与临床杂志》2020年第4期421-424,共4页Chinese Journal of Anatomy and Clinics

基　　金：安徽省教育厅高校科学研究项目(KJ2019A0372)。

摘　　要：目的探讨微小RNA (miRNA)-622对非小细胞肺癌A549细胞增殖、凋亡的影响。方法培养A549细胞,分为miRNA-622组和miRNA-622阴性对照组(NC组),分别转染miRNA-622模拟物及miRNA-622阴性对照。使用实时荧光定量聚合酶链反应(qPCR)检测miRNA-622组和NC组miRNA-622的表达水平,采用噻唑蓝实验检测两组细胞增殖能力,采用流式细胞学技术检测两组细胞凋亡率。结果 miRNA-622组和NC组细胞中miRNA-622的相对表达水平分别为0.993±0.058、0.631±0.053,OD值分别为0.631±0.049、0.922±0.058,细胞凋亡率分别为12.230%±0.176%、7.400%±0.208 %,miRNA-622组miRNA-622的相对表达水平和细胞凋亡率均高于NC组,而OD值低于NC组,差异均有统计学意义(t=23.650、17.710、3.822,P值均<0.01)。结论 miRNA-622可抑制A549细胞增殖,促进A549细胞凋亡。Objective To investigate the effect of microRNA(miRNA)-622 on the proliferation and apoptosis of non-small cell lung cancer A549 cells.Methods A549 cells were cultured and divided into miRNA-622 group and miRNA-622 negative control group(NC group)transfected with miRNA-622 mimics and miRNA-622 negative controls,respectively.MiRNA-622 expression was detected using quantitative real-time polymerase chain reaction,cell proliferation was analyzed through methyl thiazolyl tetrazolium assay,and cell apoptosis rate was examined through flow cytometry.Results The miRNA-622 and NC groups had relative miRNA-622 expression levels of 0.993±0.058 and 0.631±0.053,respectively;OD values of 0.631±0.049 and 0.922±0.058,respectively;and apoptosis rates of 12.230%±0.176%and 7.400%±0.208%,respectively.The miRNA-622 group had higher relative miRNA-622 expression and apoptotic rate but lower OD value than the NC group,and the differences were statistically significant(t=23.650,17.710,3.822,all P values<0.01).Conclusions miRNA-622 can inhibit the proliferation and promote the apoptosis of A549 cells.

关 键 词：肺肿瘤 癌 非小细胞肺 A549细胞 微小核糖核酸 增殖 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]