人牙周膜干细胞成骨分化过程中P2X7受体mRNA的表达特点及作用研究  

Expression level and effect of P2X7 receptor m RNA during osteogenic differentiation of human periodontal ligament stem cells

作　　者：李文静[1] 陈惠珍[1] 刘从娜 张志勇[1] LI Wen-jing;CHEN Hui-zhen;LIU Cong-na;ZAHNG Zhi-yong(Department of Oral Medicine,the Second Hospital of Hebei Medical University.Shijiazhuang 050000,Hebei Province,China)

机构地区：[1]河北医科大学第二医院口腔内科,河北石家庄050000

出　　处：《上海口腔医学》2020年第4期365-369,共5页Shanghai Journal of Stomatology

摘　　要：目的:探讨P2X7受体在人牙周膜干细胞成骨分化中的作用。方法:将原代培养获得的人牙周膜干细胞分为对照组、三磷酸腺苷组、成骨诱导液组和三磷酸腺苷+成骨诱导液组,比较4组的成骨分化相关基因RUNX2、OCN基因表达和P2X7受体m RNA表达的差异,采用SPSS 16.0软件包进行统计学分析。结果:成骨后1周和2周,三磷酸腺苷+成骨诱导液组的RUNX2和OCN mRNA表达显著高于成骨诱导液组(P<0.05)。三磷酸腺苷+成骨诱导液组成骨后1周的RUNX2和OCN mRNA表达显著高于成骨后2周(P<0.05)。成骨后1周和2周,三磷酸腺苷组和三磷酸腺苷+成骨诱导液组的P2X7受体mRNA表达均显著高于对照组和成骨诱导液组(P<0.05);成骨后2周,三磷酸腺苷组的P2X7受体mRNA表达显著高于三磷酸腺苷+成骨诱导液组(P<0.05);三磷酸腺苷组成骨后1周的P2X7受体mRNA表达显著高于成骨后2周(P<0.05)。结论:P2X7受体明显提高牙周膜干细胞的成骨效果,三磷酸腺苷可激活P2X7受体的表达。PURSPOSE:To investigate the role of P2 X7 receptor in osteogenic differentiation of human periodontal ligament stem cells.METHODS:Human periodontal ligament stem cells obtained from primary culture were divided into4 groups:control group,adenosine triphosphate group,osteogenic induction group,adenosine triphosphate+osteogenic induction group.The differences of RUNX2,OCN gene expression and P2 X7 receptor mRNA expression between the four groups were compared.Statistical analysis was performed with SPSS 16.0 software package.RESULTS:One week after osteogenic formation and two weeks after osteogenic formation,the expression of RUNX2 and OCN mRNA in the adenosine triphosphate+osteogenic induction group was significantly higher than that in the osteogenic induction group(P<0.05).The expression of RUNX2 and OCN mRNA in the 1 week after adenosine triphosphate+osteogenic induction fluid was significantly higher than that 2 weeks after osteogenic formation,and the difference was statistically significant(P<0.05).The expression of P2 X7 receptor mRNA in the adenosine triphosphate group and the adenosine triphosphate+osteogenic induction group was significantly higher than that in the control group and the osteogenic induction group 1 week after osteogenesis and 2 weeks after osteogenesis(P<0.05).The expression of P2 X7 receptor mRNA in the adenosine triphosphate group was significantly higher than that in the adenosine triphosphate+osteogenic induction group 2 weeks after osteogenesis(P<0.05).The expression of P2 X7 receptor mRNA was significantly higher than that of osteogenic induction 1 week after adenosine triphosphate composition(P<0.05).CONCLUSIONS:P2 X7 receptor can significantly improve the osteogenesis of periodontal ligament stem cells,and adenosine triphosphate can activate the expression of P2 X7 receptor.

关 键 词：P2X7受体 牙周膜干细胞 三磷酸腺苷 成骨分化 

分 类 号：R781.4[医药卫生—口腔医学]