胁迫条件下褪黑素诱导雨生红球藻虾青素合成  

Induced Synthesis of Astaxanthin from Haematococcus pluvialis by Melatonin under Stress

作　　者：崔静 李涛[1] 丁巍[1] 赵永腾 余旭亚[1] CUI Jing;LI Tao;DING Wei;ZHAO Yongteng;YU Xuya(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《食品与生物技术学报》2020年第6期15-21,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(21766012,21666012);云南省重大科技专项计划项目(2018ZG003);云南省健康科技计划项目(2014NS227)。

摘　　要：雨生红球藻(Haematococcus pluvialis)在胁迫条件下可大量积累虾青素,已成为天然虾青素的主要来源。通过解析外源褪黑素(Melatonin,MLT)调控雨生红球藻在缺氮联合高光照胁迫条件下的防御效应,以期建立虾青素高效合成的技术体系。结果表明,胁迫条件下外源MLT的诱导显著促进了虾青素的积累,最高质量分数达到32.37 mg/g,较对照组增加了2.25倍。此外,外源MLT提高了胞内NO和丝裂原活化蛋白激酶(MAPK)的含量,同时上调了虾青素合成关键酶基因dxs和chy的表达水平。研究表明,外源MLT诱导高光缺氮胁迫下雨生红球藻中虾青素的高效合成可能与MLT调控藻细胞内的NO、MAPK含量和虾青素合成关键酶基因dxs和chy的表达水平相关。Haematococcus pluvialis is a major source of natural astaxanthin due to its ability to accumulate astaxanthin under stress conditions.In order to establish a technical system for the regulation of efficient synthesis of astaxanthin,this experiment analyzed the effect of exogenous melatonin(MLT)on the defense response of Haematococcus pluvialis under nitrogen deficiency combined with high light condition.The results showed that the induction of exogenous MLT significantly promoted the accumulation of astaxanthin under stress conditions,with the highest content reaching 32.37 mg/g,which was 2.25 times higher than that of the control group.In addition,exogenous MLT increased the levels of intracellular NO and mitogen-activated protein kinase(MAPK),and upregulated the expression levels of dxs and chy,which are the key enzyme genes for astaxanthin synthesis.This study showed that the efficient synthesis of astaxanthin in Haematococcus pluvialis induced by exogenous MLT under high light and nitrogen stress may be related to the increase of NO and MAPK and the expression of the enzyme genes dxs and chy.

关 键 词：MLT 雨生红球藻 虾青素 NO MAPK 

分 类 号：Q81[生物学—生物工程]