外源H2S对镉胁迫下菠菜种子萌发及幼苗生长的影响  

Effects of Hydrogen Sulfide on Seed Germination and Seedling Growth of Spinach Under Cadmium Stress

作　　者：李晓科[1] 武玉珍[1] 张谨华[1] 张义贤[2] LI Xiaoke;WU Yuzhen;ZHANG Jinhua;ZHANG Yixian(School of Biology Science and Technology,Jinzhong College,Jinzhong,Shanxi 030619;School of Life Science,Shanxi University,Taiyuan,Shanxi 030006)

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030619 [2]山西大学生命科学学院,山西太原030006

出　　处：《北方园艺》2020年第16期16-21,共6页Northern Horticulture

基　　金：山西省自然科学基金资助项目(201701D121083);晋中学院博士基金资助项目(2015006)。

摘　　要：为了探明外源H2S对镉胁迫下菠菜的适应性反应特性,以菠菜为试材,采用室内培养法,研究了外源NaHS(H2S的供体)对镉胁迫下菠菜种子萌发指标的影响及幼苗的生长响应,以期为镉污染地区菠菜的栽培管理提供参考依据。结果表明:在重金属镉(0.36 mmol·L-1)和NaHS溶液(40μmol·L-1)以及H2S生成抑制剂HA溶液(0.8 mmol·L-1)处理下,与对照相比,镉和HA单独处理使菠菜种子的萌发指标(发芽率、发芽势、发芽指数、活力指数、异状发芽率)、幼苗生长指标(根长、株高、生物量、根系耐性指数)和呼吸速率显著降低。随着H2S的加入,对以上各项指标有促进作用,其中复合处理组(NaHS+Cd)表现出H2S对镉伤害的缓解作用。而萌发生理指标丙二醛(MDA)含量在镉和HA单独处理显著升高,H2S的加入使其含量下降,缓解了自由基对菠菜种子的伤害。In order to ascertain the exogenous H2 S adaptive response characteristics of spinach under Cd stress,the effects of exogenous NaHS(H2 S donor)on seed germination index and seedling growth response of spinach under Cd stress were studied by using spinach as experimental material and adopting indoor culture method,so as to provide experimental theoretical basis for planting and management of spinach in the Cd polluted areas.The results showed that in the treatment of heavy metal Cd(0.36 mmol·L-1),NaHS solution(40μmol·L-1)and H2 S generation inhibitor HA solution(0.8 mmol·L-1),compared with the control,spinach seed germination indexes(germination rate,germination potential,germination index,vigor index,unusual germination rate),seedling growth indexes(root length,plant height,biomass,root system tolerance index)and respiratory rate were reduced significantly under the treatment of Cd and HA alone.With the addition of H2 S,it had a promoting effect on the above indexes,especially the compound treatment group(NaHS+Cd)showed the mitigating effect of H2 S on Cd damage.The content of malondialdehyde(MDA),aphysiological index of germination,was significantly increased under the treatment of Cd and HA alone,while the addition of H2 S reduced MDA content and alleviated the damage of ROS to spinach seeds.

关 键 词：CD胁迫 H2S 菠菜 种子萌发 幼苗生长 

分 类 号：X171.5[环境科学与工程—环境科学]