拟南芥/本生烟远缘嫁接体中移动性mRNA分子检测  

Identification of Mobile mRNAs in Arabidopsis/Nicotiana benthamiana Hetero-grafts

作　　者：邓竹英 梁大成 DENG Zhuying;LIANG Dacheng(Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou,Hubei 434025;Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Yangtze University/Hubei Key Laboratory of Waterlogging Disaster and Wetland Agriculture,Jingzhou,Hubei 434025)

机构地区：[1]长江大学湖北省主要粮食作物产业化协同创新中心,湖北荆州434025 [2]长江大学湿地生态与农业利用教育部工程研究中心/湖北省涝渍灾害与湿地农业重点实验室,湖北荆州434025

出　　处：《北方园艺》2020年第16期22-28,共7页Northern Horticulture

基　　金：国家自然科学基金资助项目(31671257)。

摘　　要：以拟南芥和本生烟草为试材,采用微嫁接技术和转录组测序的方法,研究了拟南芥/本生烟烟草远缘嫁接体中可移动的mRNA分子,以期进一步了解根茎间的信息交流机制。结果表明:在本生烟砧木中检测出824个向下移动的拟南芥mRNA分子,挑选其中30个高丰度的拟南芥mRNA靶分子进行RT-PCR扩增验证,未发现可以移动的mRNA分子。进一步验证其中psbW和RBCS1A基因均未发生移动。利用克隆测序,检测出AT1G26630(elF5A2)的序列来自于本生烟草,而非接穗部分移动而来。深度测序得到的可移动mRNAs并未能够被RT-PCR技术所验证,上述结果表明mRNA可能并非担当根茎间长距离运输的角色。Arabidopsis and Nicotiana benthamiana were used as materials,by deploying the micrografting technique and deep-sequencing platform,the possibility of potentially mobile mRNAs between Arabidopsis scion and Nicotiana rootstock were explored in order to understand the mechanisms of root/shoot communication.The results showed that 824 transcripts belonging to Arabidopsis were identified in the Nb rootstock about deep sequencing,suggesting that mRNA might move across the graft union.To further confirm the mobility of the identified Arabidopsis transcripts,RT-PCR analyses of 30 high-potential mobile transcripts together with previously identified psbW and RBCS1 A transcripts were performed.The amplified PCR fragment for AT1 G26630(elF5 A2) gene was further cloned and sequenced,and it was from Nicotiana benthamiana rather than from the scion.Mobile mRNA candidates identified with deep sequencing technique cannot be validated by RT-PCR,thereby presenting concerns about the role of mRNAs in acting as long-distance signaling between shoot and root as previously suggested.

关 键 词：拟南芥 本生烟草 远缘嫁接 转录组测序 mRNA分子移动 

分 类 号：X173[环境科学与工程—环境科学]