鼠神经生长因子对兔眼LASIK术后角膜知觉恢复和泪液动力学指标及细胞因子的影响  

Effect of mouse nerve growth factor on corneal perceptual recovery and tear fluid dynamics indicators and cytokines after LASIK in rabbits

作　　者：曹娟[1] 马波[1] 李海燕[1] CAO Juan;MA Bo;LI Hai-yan(Department of Ophthalmology,Xi'an Fourth Hospital,Xi'an 710004,Shaanxi,China)

机构地区：[1]西安市第四医院眼科,陕西西安710004

出　　处：《川北医学院学报》2020年第4期559-562,602,共5页Journal of North Sichuan Medical College

基　　金：国家自然科学基金(81273902)。

摘　　要：目的:分析鼠神经生长因子(mNGF)对兔眼准分子激光原位角膜磨镶术(LASIK)术后角膜神经修复和角膜知觉恢复的影响。方法:随机选取3只兔子(6只眼),作为空白对照组,不做任何处理。将造模成功的60只动物(120只眼)平均分成两组,每组兔两只眼睛均接受LASIK手术,手术后治疗组(mNGF组)每日滴配制的神经生长因子(NGF)滴眼液,阴性对照组(NC组)每日滴生理盐水溶液。每日给药6次,每次1滴,每天均在同一时间滴眼。分别于术前、术后对两组兔的角膜知觉、泪液分泌量、泪膜破裂时间(BUT)、泪液中生化指标、新生神经纤维数量、上皮细胞微绒毛密度和HE染色结果等进行检测。结果:(1)角膜知觉恢复:除术后第1天外,其余各时间点mNGF组治疗效果优于NC组,且两组的差异具有统计学意义(P<0.05)。(2)泪液动力学指标检查:LASIK术后术后1周、2周、1月、3月和6月时,mNGF组兔眼泪液分泌量以及泪膜破裂时间均高于NC组,差异有统计学意义(P<0.05)。(3)泪液细胞因子检查:术后短期内NGF对TNF-α、IL-2炎性因子表达控制和对NGF促进明显优于NC组。(4)术后第1天至2周,mNGF组上皮细胞微绒毛密度明显高于NC组,两组数据差异具有统计学意义(P<0.05)。结论:(1)mNGF促进兔眼LASIK术后角膜神经上皮细胞结构恢复和角膜神经修复,其机制可能与降低炎症反应,促进NGF分泌有关。(2)mNGF局部滴眼可以改善LASIK术后干眼的症状,促进角膜知觉恢复。Objective:To analyze the effects of mouse nerve growth factor(NGF)on corneal nerve repair and corneal perception recovery after laser in situ keratomileusis(LASIK).Methods:3 rabbits(6 eyes)were randomly selected as the blank control group without any treatment.60 animals(120 eyes)were divided into two groups.Each group received LASIK.After operation,the treatment group(mNGF group)received NGF eye drops every day,while the negative control group(NC group)received normal saline solution every day.The drug was given 6 times a day,1 drop each time,and the eyes were dropped at the same time every day.The corneal perception,tear secretion,tear break-up time(but),biochemical indexes in tear,number of new nerve fibers,microvilli density of epithelial cells and the staining results were measured before and after operation.Results:(1)Corneal sensory recovery,except the first day after operation,the therapeutic effect of mNGF group was better than NC group at other time points,and the difference between the two groups was statistically significant(P<0.05).(2)Dynamics index examination in tear:at 1w,2w,1m,3m and 6m after LASIK,the amount of tear secretion and tear film rupture time in the mNGF group were higher than those in the NC group,the difference was statistically significant(P<0.05).(3)Examination of cytokine in tear:In the short term after operation,NGF could control the expression of TNF-α,IL-2 inflammatory factors and promote NGF significantly better than NC group.(4)The number of microvilli density of epithelial cells in mNGF group were significantly higher than those in NC group from the 1 d to the 2 w after operation(P<0.05).Conclusions:(1)mNGF can promote the corneal neuroepithelial cell structure and corneal nerve repair after LASIK,which may be related to the reduction of inflammatory response and the promotion of NGF secretion.(2)After LASIK,the symptom of dry eye can be improved and the recovery of corneal perception can be promoted.

关 键 词：神经生长因子 角膜 神经修复 准分子激光原位角膜磨镶术 炎症因子 

分 类 号：R779.6[医药卫生—眼科]