热化疗对口腔鳞癌患者细胞活力及癌胚抗原相关细胞黏附分子1表达的影响  

Effects of thermo chemotherapy on cell viability and expression of carcinoembryonic antigen-related cell adhesion molecule 1 in patients with oral squamous cell carcinoma

作　　者：张楠 申震[1] 张开智[2] ZHANG Nan;SHEN Zhen;ZHANG Kai-zhi(The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061;Department of Stomatology,Shangluo Central Hospital,Shangluo 726000,Shaanxi,China)

机构地区：[1]西安交通大学第一附属医院,陕西西安710061 [2]商洛市中心医院口腔科,陕西商洛726000

出　　处：《川北医学院学报》2020年第4期603-606,611,共5页Journal of North Sichuan Medical College

摘　　要：目的:探究热化疗对口腔鳞癌患者细胞活力及癌胚抗原相关细胞黏附分子1(CEACAM1)表达的的影响。方法:选取120例口腔鳞癌患者作为研究对象并设为实验组,另选取同期收治的健康体检者120名设为对照组。采用热化疗方案对口腔鳞癌进行治疗,检测并比较两组化疗前后白细胞介素2(IL-2)、肿瘤坏死因子α(TNF-α)等炎性细胞因子水平,以及CD4^+、CD8^+、CD4^+/CD8^+等T细胞亚群表达,分析两组CEACAM1表达和细胞活力情况。结果:实验组III期化疗后IL-2和TNF-α水平高于IV期,但低于对照组,差异均有统计学意义(P<0.05)。实验组III期化疗后CD4^+、CD4^+/CD8^+高于IV期,但低于对照组,CD8^+低于IV期,但高于对照组,差异有统计学意义(P<0.05)。实验组III期化疗后CEACAM-1 mRNA相对表达量和CEACAM-1蛋白阳性表达率均低于IV期,但高于对照组,差异有统计学意义(P<0.05)。实验组III期化疗后细胞活力低于IV期,差异有统计学意义(P<0.05),高于对照组但差异无统计学意义(P>0.05)。结论:热化疗应用在口腔鳞癌患者中,能够提高IL-2和TNF-α水平,增强机体免疫力,抑制细胞活力和CEACAM1表达,从而稳定患者的病情。Objective:To explore the effects of thermal chemotherapy on cell viability and expression of carcinoembryonic(CEACAM1)in patients with oral squamous cell carcinoma.Methods:120 patients with oral squamous cell carcinoma were selected as the study subjects and treated as the experimental group,while 120 healthy patients admitted to our hospital during the same period were selected as the control group.Oral squamous cell carcinoma was treated with thermochemotherapy.Inflammatory cytokines such as interleukin 2(IL-2)and tumor necrosis factor matrix(TNF-α)before and after chemotherapy were detected and compared,as well as the expression of CD4^+,CD8^+,CD4^+/CD8^+and other T cell subsets,to analyze the expression and cell activity of CEACAM1 in the two groups.Results:The level of IL-2 and TNF-αin the experimental group after stage III chemotherapy was higher than that in stage IV,but lower than that in the control group,with statistically significant differences(P<0.05).After stage III chemotherapy,CD4^+and CD4^+/CD8^+in the experimental group were higher than stage IV,but lower than that in the control group.CD8^+was lower than stage IV,but higher than that in the control group,with statistically significant differences(P<0.05).The relative expression of CEACAM-1 mRNA and positive expression rate of CEACAM 1 protein in the experimental group after stage III chemotherapy were both lower than that in stage IV,but higher than that in the control group,with statistically significant differences(P<0.05).After stage III chemotherapy,the cell activity of the experimental group was lower than that of stage IV,with a statistically significant difference(P<0.05),which was higher than that of the control group,but the difference was not statistically significant(P>0.05).Conclusion:The application of thermal chemotherapy in oral squamous cell carcinoma patients can improve the level of IL-2 and TNF-α,enhance the body immunity,inhibit the cell activity and CEACAM1 expression,so as to stabilize the patients'condition.

关 键 词：口腔鳞癌 热化疗 炎性因子 免疫功能 细胞活力 CEACAM1 

分 类 号：R739.8[医药卫生—肿瘤] R363.2[医药卫生—临床医学]