ProGRP联合NUSAP1在高原地区非小细胞肺癌患者中预后的检测价值  

ProGRP combined with NUSAP1 in the prognostic value of non-small cell lung cancer patients in plateau areas

作　　者：杨兴唐 马秀清[1] 马慧英[1] 贾军[1] 秦慧萍 高兴娟[1] YANG Xing-tang;MA Xiu-qing;MA Hui-ying;JIA Jun;QIN Hui-ping;GAO Xing-juan(Department of Clinical Laboratory,Qinghai Red Cross Hospital,Xining 810000,Qinghai,China)

机构地区：[1]青海红十字医院检验科,青海西宁810000

出　　处：《川北医学院学报》2020年第4期607-611,共5页Journal of North Sichuan Medical College

基　　金：青海省科技项目(2015-ZJ-766)。

摘　　要：目的:探讨促胃泌素释放肽前体(ProGRP)联合核仁纺锤体相关蛋白1(NUSAPl)在高原地区非小细胞肺癌(NSCLC)患者中预后的检测价值。方法:88例高原地区确诊为NSCLC的患者,在新辅助化疗后行手术治疗。随访3年,根据患者生存情况分为生存组与非生存组,采用ELISA法(酶联免疫吸附法)ProGRP水平,采用实时荧光定量PCR检测NUSAPl mRNA水平,采用免疫组化法检测癌组织NUSAPl表达,分析其与患者预后的相关性。结果:治疗后存活组与未存活组不同病理类型患者ProGRP水平均较治疗前降低(P<0.05)。存活组腺癌NUSAP1 mRNA表达低于鳞癌(P<0.05);腺癌患者NUSAP1高表达率低于鳞癌患者(P<0.05);存活组腺癌患者NUSAP1 mRNA表达低于未存活组(P<0.05),且NUSAP1高表达率低于未存活组(P<0.05)。NUSAP1单独检测肺腺癌的AUC面积为0.727,ProGRP联合NUSAP1检测的AUC面积为0.770。结论:高原地区NSCLC患者治疗后ProGRP水平明显降低,NUSAP1 mRNA水平升高及癌组织NUSAP1高表达的患者生存率更低,NUSAP1与肺腺癌患者预后关系更为密切,可作为肺腺癌患者潜在的预后标记物。Objective:To explore the prognostic value of gastrin-releasing peptide precursor(ProGRP)combined with nucleolar spindle-associated protein 1(NUSAPl)in patients with non-small cell lung cancer(NSCLC)in plateau regions.Methods:88 patients diagnosed with NSCLC in the plateau region were selected as the research objects,and all patients underwent surgery after neoadjuvant chemotherapy.Follow-up for 3 year,divided into survival group and non-survival group according to the survival status of patients.ProGRP levels were measured by ELISA(enzyme-linked immunosorbent assay),NUSAPl mRNA level was detected by real-time fluorescent quantitative PCR,and NUSAPl expression was detected by immunohistochemistry,and the correlation between prognosis and prognosis was analyzed.Results:ProGRP levels of patients with different pathological types in the surviving group and the non-surviving group were lower than those before treatment(P<0.05).The expression of NUSAP1 mRNA in adenocarcinoma in surviving group was lower than that in squamous cell carcinoma(P<0.05),the high expression rate of NUSAP1 in patients with adenocarcinoma was lower than that in squamous cell carcinoma(P<0.05).The expression of NUSAP1 mRNA in survival group was lower than that in non survival group(P<0.05),and the high expression rate of NUSAP1 was lower than the non-surviving group(P<0.05).The AUC area detected by NUSAP1 alone for lung adenocarcinoma was 0.727,and the AUC area detected by ProGRP combined with NUSAP1 was 0.770.Conclusion:ProGRP levels of NSCLC patients in plateau areas are significantly reduced.Patients with elevated NUSAP1 mRNA levels and cancer tissues with high expression of NUSAP1 have lower survival rates,and NUSAP1 is more closely related to the prognosis of lung adenocarcinoma patients,which can be used as a potential prognostic marker for lung adenocarcinoma patients.

关 键 词：促胃泌素释放肽前体 核仁纺锤体相关蛋白1 高原地区 非小细胞肺癌 预后 

分 类 号：R734.2[医药卫生—肿瘤]