金属离子胁迫对钴酸锂生物浸出过程影响研究  

Effects of metal ion stress on bioleaching processes of lithium cobalt oxide

作　　者：刘浩 刘德洪 程国庆 张旭[1] 顾停月[2] 朱明龙[1] 谭文松[1] 吴星莹 LIU Hao;LIU De-hong;CHENG Guo-qing;ZHANG Xu;GU Ting-yue;ZHU Ming-long;TAN Wen-song;WU Xing-ying(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;Department of Chemical and Biomolecular Engineering,The Ohio State University,Athens OH 45701,America)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]美国俄亥俄大学化学与生物分子工程系,Athens OH 45701

出　　处：《高校化学工程学报》2020年第4期954-962,共9页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(21878083)。

摘　　要：生物浸出过程中冶金微生物构成的生物被膜结构与生物浸出效率之间存在密切关系。针对生物浸出过程存在料浆浓度限制问题,实验探究了生物浸出钴酸锂过程效率特征及Li^+、Co^2+胁迫对生物被膜的结构、生物量、死、活黏附菌分布和胞内活性氧簇(ROS)含量等特征的影响,结果表明钴酸锂浸出效率随料浆浓度增加而降低,并且随浸出体系中Li^+、Co^2+浓度增加,黏附菌胞内ROS水平迅速上升,黏附菌分布稀疏并大量死亡。外源添加N-乙酰-L-半胱氨酸(NAC)能缓解Li^+、Co^2+胁迫下黏附菌胞内ROS迅速升高的情况,维持生物被膜内黏附菌的活性,最终提高了5.0%(w×v^-1)钴酸锂料浆浓度下的生物浸出效率。Biofilms formed by metallurgical microorganisms are closely related to bioleaching efficiency.In order to solve the problem of slurry concentration limitation in bioleaching,efficiency characteristics of bioleaching processes of lithium cobalt oxide,effects of Li^+,Co^2+stress on the biofilm structure,biomass,distribution of dead and live sessile bacteria,and the content of intracellular reactive oxygen species(ROS)were investigated.The results show that the bioleaching efficiency of lithium cobalt oxides decreases as the slurry concentration increases,and the content of intracellular ROS in sessile bacteria increases rapidly with the increase of Li^+,Co^2+concentration in the bioleaching system.Sessile bacteria distribute sparsely and most of them were dead.Exogenous addition of N-acetyl-L-cysteine(NAC)can alleviate the rapid increase of intracellular ROS level in sessile bacteria under Li^+,Co^2+stress,maintain the activity of sessile bacteria in biofilm,and finally improve the bioleaching efficiency at 5.0%(w×v^-1)lithium cobalt oxide slurry concentration.

关 键 词：生物被膜 金属离子胁迫 生物浸出 ROS NAC 钴酸锂 

分 类 号：TQ465.92[化学工程—制药化工]