抑制DLK1表达对食管癌细胞放射敏感度的影响  

Effect of Inhibiting DLK1 Expression on Radiosensitivity of Esophageal Cancer Cells

作　　者：何翠 陈春丽 柯青 沈力 邓鑫州 顾兵[4] 孙蕊格 段奇文 HE Cui;CHEN Chunli;KE Qing;SHEN Li;DENG Xinzhou;GU Bing;SUN Ruige;DUAN Qiwen(Postgraduate Training Basement of Jinzhou Medical University,Taihe Hospital,Hu bei University of Medicine,Shiyan 442000,China;Cancer Prevention and Treatment Center,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China;Department of Biochemistry,School of Basic Medical Sciences,Hubei University of Medicine,Shiyan 442000,China;Department of Oncology,The First Hospital of Danjiangkou City,Danjiangkou 442700,China)

机构地区：[1]锦州医科大学十堰市太和医院研究生培养基地(湖北医药学院附属医院),十堰442000 [2]湖北医药学院附属十堰市太和医院肿瘤防治中心,十堰442000 [3]湖北医药学院基础医学院生物化学教研室,十堰442000 [4]丹江口市第一医院肿瘤科,丹江口442700

出　　处：《肿瘤防治研究》2020年第8期578-582,共5页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(81602391,81802997,81502666);湖北省自然科学基金(2018CFB405,2017CFB456,2017CFB167);湖北省教育厅基金(D20172102,Q20162109)。

摘　　要：目的探讨抑制DLK1的表达对食管癌细胞放射敏感度的影响。方法采用克隆形成实验验证KYSE-R与KYSE细胞的放射敏感度差异,RT-PCR和Western blot检测DLK1的表达。选用最佳DLK1干扰序列转染KYSE-R细胞,4 Gy射线处理后克隆形成实验检测KYSE-R细胞放射敏感度,流式细胞术检测细胞凋亡和细胞周期。结果KYSE-R细胞表现出比KYSE细胞更强的放射抗拒性,DLK1在KYSE-R中的表达显著高于KYSE,且siDLK1-3干扰效果最佳(均P<0.05)。4 Gy射线照射后DLK1干扰组细胞存活力显著低于对照组,而DLK1干扰组细胞凋亡率和G2/M期细胞比例明显高于对照组(均P<0.05)。结论抑制DLK1表达可通过降低细胞存活、促进细胞凋亡和增加G2/M期细胞比例、增强放射敏感度,为食管癌放射增敏研究提供新的靶点。Objective To investigate the effect of inhibiting DLK1 expression on the radiosensitivity of esophageal cancer cells.Methods The difference in radiosensitivity between KYSE-R and KYSE cells was verified by colony formation assay.RT-PCR and Western blot were used to detect DLK1 expression.The best DLK1 interference sequence was used to transfect KYSE-R cells.After 4Gy radiation treatment,clone formation experiment was used to detect the radiosensitivity of KYSE-R cells,and flow cytometry was used to detect apoptosis and cell cycle.Results KYSE-R cells showed stronger radioresistance than KYSE cells.The expression of DLK1 in KYSE-R cells was significantly higher than that in KYSE cells,and the interference effect of siDLK1-3 was the best(all P<0.05).Compared with the control group,the cell viability was significantly lower after 4Gy irradiation,while the apoptosis rate and cell proportion in G2/M phase were significantly higher in DLK1 interference group(all P<0.05).Conclusion The inhibition of DLK1 expression could provide a new target for radiosensitization research of esophageal cancer by reducing cell survival,promoting apoptosis,increasing the cell proportion in G2/M phase and enhancing radiosensitivity.

关 键 词：DLK1 食管癌 放射敏感度 

分 类 号：R735.1[医药卫生—肿瘤]