花椒精油对1型糖尿病小鼠肠道健康的影响  

Effects of Essential Oil from Zanthoxylum bungeanum on Intestinal Health of TypeⅠDiabetic Mice

作　　者：任廷远 陆敏涛 黄涛 陆龙发 杨建 秦礼康 Ren Tingyuan;Lu Mintao;Huang Tao;Lu Longfa;Yang Jian;Qin Likang(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025;Guizhou Xuande Biotechnology Co.,Ltd.,Guiyang 550016;College of Life Sciences,Guizhou University,Guiyang 550025;Guiyang Station for DUS Testing Center of New Plant Varieties of the Ministry of Agriculture and Rural Affairs of the People′s Republic of China in Guizhou Academy of Agricultural Sciences,Guiyang 550006)

机构地区：[1]贵州大学酿酒与食品工程学院,贵阳550025 [2]贵州玄德生物科技股份有限公司,贵阳550016 [3]贵州省农业生物工程重点实验室,贵阳550025 [4]贵州省农业科学院农业部DUS中心贵阳分中心,贵阳550006

出　　处：《中国粮油学报》2020年第8期88-96,共9页Journal of the Chinese Cereals and Oils Association

基　　金：贵州省科技计划(黔科合平台人才[2018]5781-50);贵州大学引进人才科研项目[贵大人基合字(2018)42号];毕节市科技项目(毕科联合字ZY[2017]01号)。

摘　　要：为了探索花椒精油对1型糖尿病小鼠肠道健康的影响,将造模成功的40只雄性小鼠,按体质量和血糖随机分为模型组、花椒精油高剂量组15 mg/(kg·d)、中剂量组9 mg/(kg·d)和低剂量组3 mg/(kg·d),每组10只,饲养28 d。实验结束后,检测盲肠壁表面积、pH值、游离氨、盲肠微生物和短链脂肪酸等。实验结果表明,与模型组相对,花椒精油可显著(P<0.05)增加小鼠体质量和饲料效率,降低其采食量;可显著(P<0.05)增加盲肠内容物中短链脂肪酸的含量;可显著(P<0.05)增加盲肠内容物乳酸杆菌和双歧杆菌的量;高、中剂量可显著降低肠杆菌和肠球菌的量。结果表明,花椒精油可促进因STZ诱导的1型糖尿病小鼠盲肠内容物中有益菌的生长,抑制有害菌的生长,从而改善1型糖尿病小鼠的肠道健康。Previous studies have indicated that Zanthoxylum bungeanum and its flavored ingredients had an impact on the intestinal health.However,the impact of Essential Oil fromZanthoxylum bungeanumon intestinal health is still rarely involved.To clarify the effects of essential Oil from Zanthoxylum bungeanum on the intestinal health of typeⅠdiabetic mice,a total of 40 streptozotocin(STZ)-induced typeⅠdiabetic male mice were randomly divided into four groups according to body weight and blood glucose:model group,high[15 mg/(kg·d)],middle[9 mg/(kg·d)],and low[3 mg/(kg·d)]dose essential oil groups.After 28 days of administration,the area of cecum wall surface,pH value and free ammonia of cecum content,cecum microflora and fatty acids were measured.The results indicated that essential oil significantly increased the body weight and feed efficiency,and reduced feed intake(P<0.05)when compared with the control,essential oil also significantly increased amount of short-chain fatty acids(SCFAs),lactobacilli and bifidobacteria in cecum contents(P<0.05).Importantly,middle and high doses of essential oil significantly reduced the amount of enterobacteria and enterococci in cecum contents(P<0.05).These results indicated that essential oil fromZanthoxylum bungeanum can promote the growth of beneficial bacteria and inhibit the growth of harmful bacteria in cecum contents of STZ-induced typeⅠdiabetic mice,finally improve the intestinal health of typeⅠdiabetic mice.

关 键 词：花椒精油 糖尿病 肠道微环境 肠道微生物 

分 类 号：R285.5[医药卫生—中药学]