马铃薯乙醇酸氧化酶StGLO1对模拟水分胁迫的响应  

Response of glycolate oxidase StGLO1 to simulated water stress in potato(Solanum tuberosum L.)

作　　者：王芳[1,2,3,4] 杨振红 赵龙[1] WANG Fang;YANG Zhenhong;ZHAO Long(Academy of Agricultural and Forestry,Qinghai University,Xining 810016,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China;Key Laboratory of Qinghai-Tibetan Plateau Biotechnology of Ministry of Education,Xining 810016,China;Key Laboratory of Potato Breeding of Qinghai,Xining 810016,China)

机构地区：[1]青海大学农林科学院,青海西宁810016 [2].青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810016 [3]青藏高原生物技术教育部重点实验室,青海西宁810016 [4]青海省马铃薯育种重点实验室,青海西宁810016

出　　处：《青海大学学报》2020年第4期19-26,33,共9页Journal of Qinghai University

基　　金：国家马铃薯产业技术体系项目(CARS-09);青海省科学技术厅重大专项(2019-NK-A1)。

摘　　要：为了解乙醇酸氧化酶在调控植物生长代谢及生物与非生物胁迫过程中发挥的作用。本研究以‘青薯9号’马铃薯为材料克隆乙醇酸氧化酶基因StGLO1,构建过表达载体并转化烟草,观察在干旱胁迫处理下转基因烟草和野生型烟草的长势,并测定了叶片中的游离脯氨酸、CAT、POD的含量以及转基因根茎叶中StGLO1基因的表达量变化,验证过表达StGLO1烟草在干旱胁迫下的响应。结果表明:StGLO1(GenBank登录号XM006348318.2)基因CDS为1116 bp,编码371个氨基酸;干旱胁迫下,转StGLO1基因烟草的生长优于野生型,转基因烟草StGLO1基因的表达量在叶片中最高;转基因烟草中的游离脯氨酸、CAT、POD的含量高于野生型,达到差异显著水平(P<0.05)。该结果为进一步研究马铃薯干旱胁迫响应机制有一定参考价值,也丰富了马铃薯抗旱育种的基因资源。In order to understand the role of glycolate oxidase in the regulation of plant growth and metabolism and the process of biotic and abiotic stress.In this experiment,we cloned glycolate oxidase gene StGLO1 from‘Qingshu 9’potato,construct overexpression vector and transform tobacco,observe the growth of transgenic tobacco and wild-type tobacco under drought stress,and determine the content of free proline,CAT,POD in leaves,as well as the change of StGLO1 gene expression in roots,stems and leaves of transgenic tobacco.The results show that StGLO1(GenBank accession XM006348318.2)gene is 1116 bp,encoding 371 amino acids.Under drought stress,the growth of transgenic tobacco with StGLO1 gene is better than that of wild type,and the expression of StGLO1 gene in transgenic tobacco is the highest in the leaves.The content of free proline,CAT and POD in transgenic tobacco is higher than that of wild type,reaching a significant level(P<0.05).Our research provided the valuable information for understanding the drought resistance mechanism of potato,and enriching the genetic resources of drought resistant potato breeding.

关 键 词：马铃薯 乙醇酸氧化酶 干旱胁迫 转基因烟草 

分 类 号：S532[农业科学—作物学]