洋甘菊提取物对左旋硝基精氨酸诱导高血压大鼠血压的影响及机制研究  

Study on the effect of Matricaria chamomilla extract on blood pressure in treating NG-Nitro-L-arginine hypertension rats and its mechanism

作　　者：罗晶晶[1] 姚雪萍[1] 轩然 刘备 于亚洲 孙湛 LUO Jing-Jing;YAO Xue-Ping;XUAN Ran;LIU Bei;YU Ya-Zhou;SUN Zhan(Functional Center,School of Basic Medical Sciences,Xinjiang Medical University,Urumqi 830011,China;Clinical Medical School,Xinjiang Medical University,Urumqi 830011,China;Department of Pathophysiology,School of Basic Medical Sciences,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学基础医学院机能中心,乌鲁木齐830011 [2]新疆医科大学临床医学院,乌鲁木齐830011 [3]新疆医科大学基础医学院病理生理学教研室,乌鲁木齐830011

出　　处：《食品安全质量检测学报》2020年第16期5719-5723,共5页Journal of Food Safety and Quality

基　　金：新疆医科大学科研创新项目(XYDCX201607);新疆维吾尔自治区自然科学基金项目(2019D01C207)。

摘　　要：目的研究洋甘菊提取物对左旋硝基精氨酸(NG-Nitro-L-arginine,L-NNA)诱导高血压大鼠血压的影响及作用机制。方法给L-NNA药8周复制高血压大鼠模型,将高血压大鼠随机分为模型组、洋甘菊提取物低、中和高剂量组(62.5、125和250mg/kg)及卡托普利组(25mg/kg),正常SD大鼠作为正常对照组,每组8只。给药8周,每周测定血压。8周后,测定血清血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、血管紧张素1-7(angiotensin-(1-7), Ang1-7)、超氧化物歧化酶(superoxide dismutase, SOD)、胱抑素C(cystatin C, CysC)和一氧化氮(nitricoxide,NO),同时HE染色观察心脏和肾脏病理组织形态学的变化。结果治疗前后对比,洋甘菊提取物中剂量组和高剂量组大鼠收缩压均呈降低趋势(P<0.05);与模型组相比,洋甘菊提取物高剂量组和卡托普利组大鼠的AngⅡ含量降低(P<0.05);SOD明显升高(P<0.01);Cys-C含量降低(P<0.05);卡托普利组大鼠的Ang1-7含量明显升高(P<0.01);各组NO含量有一些增高,但无统计学意义(P?0.05)。HE染色明显看出治疗组能够缓和心脏和肾脏的损伤现象。结论洋甘菊提取物对L-NNA诱导的高血压大鼠血压有一定的降低作用,对心、肾组织有保护作用,其降压机制可能与降低AngⅡ的含量,改善氧化应激有关。Objective To investigate the effect and mechanism of Matricaria chamomilla extract on blood pressure in treating NG-Nitro-L-arginine hypertension rats. Methods Hypertensive rats were given NG-Nitro-L-arginine(L-NNA) for 8 weeks to produce hypertensive model, The hypertensive rats were randomly divided into groups:model group, low(62.5 mg/kg), middle(125 mg/kg) and high(250 mg/kg) dose group of Matricaria chamomilla extract;captopril(25 mg/kg);each group was given a continuous gavage for 8 weeks, and normal SD rats were used as normal control group, with 8 rats in each group. The arterial blood pressure was measured once a week. After eight weeks of treatment, the content of angiotensin Ⅱ(Ang Ⅱ), angiotensin-(1-7)(Ang-(1-7)), superoxide dismutase(SOD), cystatinC(CysC) and nitric oxide(NO) in blood was measured. The changes of heart and kidney structure were observed by HE staining. Results Compared with before and after treatment, the systolic blood pressure of middle and high dose group decreased(P<0.05);Compared with the model group, the content of AngⅡ in the high dose and captopril group were decreased(P<0.05);SOD increased significantly(P<0.01);Cys-C content decreased(P<0.05);The content of Ang1-7 in captopril group was significantly increased(P<0.01);The content of NO in each group increased, but there was no statistical significance(P<0.05). HE staining showed that the treatment group could alleviate the damage of heart and kidney. Conclusion Matricaria chamomilla extract can reduce the blood pressure of hypertensive rats induced by L-NNA. It has a protective effect on heart and renal, the antihypertensive mechanism of Matricaria chamomilla extract may be related to the decrease of AngⅡ content and improve oxidative stress.

关 键 词：洋甘菊提取物 高血压大鼠 降压 药效学 

分 类 号：R285.5[医药卫生—中药学]