吡哆醛激酶(PDXK)促进浆液性卵巢癌细胞增殖并与不良预后相关  

Pyridoxal kinase (PDXK) promotes the proliferation of serous ovarian cancer cells and is associated with poor prognosis

作　　者：谭魏 刘斌[2] 令狐华[1] TAN Wei;LIU Bin;LING Huhua(Department of Obstetrics and Gynecology,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Department of Pathology,Basic Medical School,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆4000162 [2]重庆医科大学基础医学院病理科,重庆400016

出　　处：《细胞与分子免疫学杂志》2020年第6期542-548,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81572562)。

摘　　要：目的探讨吡哆醛激酶(PDXK)对浆液性卵巢癌(SOC)细胞株HEY细胞增殖的影响,分析PDXK表达水平与SOC患者临床病理特征及生存之间的相关性。方法通过实时定量PCR、Western blot法检测PDXK在IOSE80正常卵巢细胞株与3AO、A2780、OVCAR3、ES-2、HEY卵巢癌细胞的表达水平。在高表达PDXK的HEY卵巢癌细胞敲低PDXK,用CCK-8检测细胞增殖,流式细胞术检测细胞周期。免疫组织化学染色法检测PDXK在正常卵巢上皮组织及SOC组织中的表达,分析PDXK表达与SOC患者临床特征及预后的关系。结果PDXK在卵巢癌细胞株中表达高于正常卵巢细胞株,敲低PDXK可以抑制HEY细胞的增殖,阻滞细胞周期由G1期向S期转化。PDXK在SOC组织中表达高于正常卵巢组织,且与卵巢癌的FIGO晚期、肿瘤低分化、多糖抗原125(CA125)表达呈正相关,与更短的无进展生存(PFS)时间相关。结论PDXK促进SOC细胞增殖,与临床预后差相关。Objective To explore the effects of pyridoxal kinase(PDXK)on the proliferation of serous ovarian cancer(SOC)HEY cells,and analyze its relationship with clinicopathological features of patients.Methods The expression of PDXK in normal ovarian IOSE80 cells and ovarian cancer 3AO,A2780,OVCAR3,ES-2,HEY cells were detected by real-time quantitative PCR and Western blotting.After the knockdown of PDXK in ovarian cancer HEY cells,CCK-8 assay was used to detect cell proliferation and flow cytometry was used to detect cell cycle.Furthermore,we detected the expression of PDXK in normal ovarian epithelial tissues and SOC tissues by immunohistochemistry,and analyzed its correlation with clinicopathological features and prognosis.Results The expression of PDXK in ovarian cancer cell lines was higher than that in normal ovarian cell lines.Knockdown of PDXK attenuated the proliferation and blocked the transition of G1/S phase in HEY cells.PDXK expression was higher in SOC tissues than in normal ovarian tissues.Higher PDXK expression was positively correlated with advanced FIGO stage,poor differentiation and higher CA125 level.Besides,patients with higher PDXK expression had shorter progression-free-survival(PFS).Conclusion PDXK may promote SOC cell proliferation and be associated with a poor prognosis.

关 键 词：吡哆醛激酶 卵巢癌 细胞增殖 临床病理特征 

分 类 号：R737.31[医药卫生—肿瘤] R392-33[医药卫生—临床医学]