联合应用骨形成蛋白-2与转化生长因子-1α对比格犬上颌窦外提升术中成骨能力的影响  

Effects of Combined Application of BMP-2 and TGF-1αon Bone Formation in Maxillary Sinus Lifting in Dogs

作　　者：马冬 刘鑫[1] 刘亚军 丁如愿 杨刚 陈永翔 王元银[1] MA Dong;LIU Xin;LIU Yajun;DING Ruyuan;YANG Gang;CHEN Yongxiang;WANG Yuanyin(Department of Oral Implantology, Oral and Maxillofacial Center, Stomatologic Hospital & College, Anhui Medical University, Key Lab. of Oral Diseases Research of Anhui Province, Hefei 230032, China)

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,安徽合肥230032

出　　处：《口腔医学研究》2020年第9期850-854,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81271162);安徽省自然科学基金(编号:1708085MH194)。

摘　　要：目的:体外实验检测骨形成蛋白-2(BMP2)与转化生长因子-1α(TGF-1α)对犬骨髓干细胞(BMSCs)骨向分化的影响,并通过体内实验验证。方法:通过构建慢病毒过表达BMP2与TGF-1α载体,复合BIOSS骨粉植入犬上颌窦,检测新生骨形成量。结果:联合应用BMP2与TGF-1α侧的上颌窦成骨量明显高于其他组,并获得更高的生物学稳定性。结论:BMP2与TGF-1α的使用可明显促进骨形成,具有临床应用前景。Objective:To test the effects of BMP2 and TGF-1αon bone differentiation of canine bone marrow stem cells in vitro,and verify it in vivo.Methods:BMP2 and TGF-1αwere overexpressed by constructing lentivirus,and then combined with BIOSS bone meal and implanted into the maxillary sinus of dogs.New bone formation and biological stability(by ISQ)were detected.Results:Bone formation in the combined application of BMP2 and TGF-1αgroup was significantly higher than those of other groups,and the same as biological stability.Conclusion:The application of BMP2 and TGF-1αcan significantly promote bone formation,which has clinical application prospects.

关 键 词：骨形成蛋白-2 转化生长因子-1α 复合材料 上颌窦 

分 类 号：R78[医药卫生—口腔医学]