网膜包裹对骨髓间充质干细胞与膀胱脱细胞基质管状移植物的促血管化作用  

Pro-vascularization of tubular graft of bone marrow mesenchymalstem cells and bladder acellular matrix wrapped up in omentum

作　　者：李欣慧 喻雄杰 俞远东 张军 吴光 LI Xinhui;YU Xiongjie;YU Yuandong;ZHANG Jun;WU Guang(Cancer Center,Renmin Hospital of Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属人民医院肿瘤中心,湖北十堰442000

出　　处：《西安交通大学学报（医学版）》2020年第5期706-712,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.31400835);湖北省卫健委基金资助项目(No.WJ2019Q013)。

摘　　要：目的探讨网膜包裹对骨髓间充质干细胞-膀胱脱细胞基质(BMSCs-BAM)管状移植物的血管化作用。方法将体外培养的BMSCs种植于已制备的BAM支架上,进而制作成管状移植物。选取30只健康新西兰白兔,随机分为3组,每组10只,实验组:网膜包裹BMSCs-BAM管状移植物;对照组:网膜包裹BAM管状移植物;空白组:网膜包裹硅胶导管。分别于移植后的第4周和第8周取材,Western blotting和免疫组化技术检测移植物的血管内皮生长因子(VEGF)蛋白、CD34表达及微血管密度。结果①实验组VEGF蛋白表达高于对照组和空白组,对照组VEGF蛋白表达高于空白组,三组间差异均具有统计学意义[F(2,15)=5.314,P=0.017];②实验组CD34表达阳性的微血管数量高于对照组和空白组,且血管之间连接更紧密,同时对照组CD34表达阳性的微血管数量也较空白组多;③实验组的微血管密度高于对照组和空白组,对照组的微血管密度高于空白组,三组间差异均具有统计学意义[F(2,12)=6.172,P=0.021]。结论本研究证实网膜包裹可促进BAM的血管化,且将BMSCs种植于BAM上行网膜包裹培养,更有利于移植物的血管化。Objective To study the vascularization of tubular graft of bone marrow mesenchymal stem cells(BMSCs)and bladder acellular matrix(BAM)wrapped up in omentum.Methods We planted BMSCs on the BAM and made it into tubular grafts.Thirty healthy New Zealand white rabbits were randomly divided into three groups with 10 rabbits in each.Rabbits in experimental group received BMSCs-BAM tubular graft wrapped in omentum.Rabbits in control group received BAM tubular graft wrapped in omentum.Rabbits in blank group were planted with silicone catheter wrapped in omentum.Vascular endothelial growth factor(VEGF)protein,CD34 expression and microvessel density of the graft were measured at weeks 4 and 8,respectively.Results①The expression of VEGF protein was higher in the experimental group than in the control group and the blank group,and the expression of VEGF protein was higher in the control group than in the blank group;the differences among the three groups were statistically significant[F(2,15)=5.314,P=0.017].②The microvessels with positive CD34 expression were denser in the experimental group than in the other two groups,and the blood vessels were closely connected with each other.The microvessels with positive CD34 expression were also much denser in the control group than in the blank group.③The microvessel density was higher in the experimental group than in the control group and the blank group,and it was also higher in the control group than in the blank group.The differences among the three groups were statistically significant[F(2,12)=6.172,P=0.021].Conclusion Omental wrapping promotes vascularization of BAM,and planting BMSCs on the BAM is more conducive to vascularization of BAM.

关 键 词：骨髓间充质干细胞 膀胱脱细胞基质 网膜包裹 血管化 

分 类 号：R394.2[医药卫生—医学遗传学]