生物转化脂肪酸合成ω-羟基酸和ω-氨基酸研究进展  

Advances in biosynthesis of fatty acids to ω-hydroxyacids and ω-amino acids

作　　者：丁良怡 种刚刚 潘江[1] 许建和[1] DING Liangyi;CHONG Ganggang;PAN Jiang;XU Jianhe(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《化工学报》2020年第9期3919-3932,共14页CIESC Journal

基　　金：国家自然科学基金项目(21536004,21776085);国家重点研发计划项目(2019YFA09005000,2018YFC1706204)。

摘　　要：ω-羟基脂肪酸和ω-氨基脂肪酸可广泛用于聚酯、聚酰胺等高分子的合成以及润滑油、生物燃料、医药中间体等化工产品的生产。近年来,以自然界丰富的可再生资源脂肪酸为绿色原料,生产这类可降解的生物基材料及化学品引起了人们的广泛兴趣,其中从植物油中的油酸、亚油酸等脂肪酸出发生产中链脂肪酸及其羟基、氨基衍生物的非天然生物催化反应也越来越丰富,是合成生物学技术在油脂资源转化方面的一个重要应用。综述了近年来中链ω-羟基脂肪酸与ω-氨基脂肪酸生物合成的研究进展,展示了通过多酶级联催化反应将可再生的脂肪酸生物转化,可持续生产9-羟基壬酸、6-氨基己酸、ω-氨基十二烷酸等高附加值精细化学品的合成路径和应用前景。ω-Hydroxy fatty acids and ω-amino fatty acids can be widely used in the synthesis of polymers such as polyester and polyamide,as well as the production of chemical products such as lubricants,biofuels,and pharmaceutical intermediates.Using of the most abundant natural fatty acids as raw materials to produce fatty acid derivatives has caused extensive researches.In recent years,the biosynthetic reactions for producing medium-chain fatty acid derivatives have become more abundant.This article reviews the research progress in biosynthesis of midchain ω-hydroxy fatty acids and ω-amino fatty acids in recent years,indicating the synthetic routes and application potential for the sustainable production of value-added fine chemicals such as 9-hydroxynonanoic acid,6-aminocaproic acid,and ω-aminododecanoic acid using multi-enzyme cascades.

关 键 词：ω-氨基脂肪酸 生化工程 多酶级联反应 催化剂 酶 ω-羟基脂肪酸 植物油 

分 类 号：Q814.9[生物学—生物工程]