硼砂对斑马鱼发育的影响  

The effect of borax on the development of zebrafish

作　　者：金鑫 周钰林 陈锡强[2] 张立涛 葛顺 JIN Xin;ZHOU Yulin;CHEN Xiqiang;ZHANG Litao;GE Shun(Jining Medical University,Rizhao 276826,China;Biology Institute,Shandong Academy of Sciences,Jinan 250014,China)

机构地区：[1]济宁医学院生物科学学院,日照276826 [2]山东省科学院生物研究所,济南250014

出　　处：《济宁医学院学报》2020年第4期229-232,共4页Journal of Jining Medical University

基　　金：济宁医学院大学生创新训练计划项目(CX2018035)。

摘　　要：目的以野生型和肝脏转基因T3斑马鱼为研究对象,探究硼砂对斑马鱼的发育毒性。方法不同浓度的硼砂分别处理斑马鱼胚胎,以受精后小时(hpf)为时间段,每24h观察并统计野生型斑马鱼的自主活动次数、孵化率、心率、畸形率、死亡率以及肝脏发育面积。结果斑马鱼胚胎24hpf,随硼砂浓度升高,硼砂各浓度处理组自主活动次数逐渐降低,0.6mg/ml硼砂组与空白对照组差异具有统计学差异(P<0.01)。与空白对照组相比,斑马鱼胚胎72hpf和96hpf,硼砂各浓度处理组斑马鱼心率显著降低(P<0.01);96hpf,0.9mg/ml硼砂组的畸形率显著性升高(P<0.01);96hpf和120hpf,0.9mg/ml硼砂组的死亡率显著性升高(P<0.01);120hpf和144hpf,0.9mg/ml硼砂组斑马鱼幼鱼的肝脏面积显著减小(P<0.01)。结论高浓度的硼砂对斑马鱼具有发育毒性。Objective To study the developmental toxicity of borax to zebrafish of wild and liver transgenic T3(lfabp:EGFP).Methods Zebrafish embryo were treated with different concentrations of borax.The number of spontaneous movement,hatching rate,heart rate,malformation,mortality and liver area of zebrafish were observed and counted every 24 hours.Results When zebrafish eggs were fertilized for 24 hours,the number of spontaneous movement gradually was decreased with the concentrations of borax increased.There were statistical difference when the concentrations of borax reached 600mg/L(P<0.01).When zebrafish eggs were fertilized for 72 and 96 hours,the heart rate in borax group significantly was decreased(P<0.01).When zebrafish eggs were fertilized for 96 hours,the malformation in borax group(900mg/L)was significantly increased(P<0.01).When zebrafish eggs were fertilized for 96 and 120 hours,the mortality in borax group(900mg/L)was sharply increased(P<0.01).When zebrafish eggs were fertilized for 120 and 144 hours,the liver area of zebrafish juvenile in borax group(900mg/L)significantly decreased(P<0.01).Conclusion High concentrations of borax had developmental toxicity for zebrafish.

关 键 词：硼砂 斑马鱼 发育毒性 

分 类 号：R22[医药卫生—中医基础理论] R285.5[医药卫生—中医学]