依普黄酮对骨质疏松成骨细胞增殖分化能力影响的研究  

Effects of ipriflavone on proliferation and differentiation of osteoporotic osteoblasts in osteoporosis

作　　者：于书娟 刘洪臣[2] YU Shu-juan;LIU Hong-chen(Stomatology Department,960th Hospital of PLA,Shandong 250031,China)

机构地区：[1]解放军第九六○医院口腔科,山东250031 [2]解放军总医院口腔医学研究所,口腔颌面修复军队医学重点实验室,北京100853

出　　处：《中华老年口腔医学杂志》2020年第4期200-203,共4页Chinese Journal of Geriatric Dentistry

摘　　要：目的:本研究旨在探讨依普黄酮对成年骨质疏松大鼠下颌骨成骨细胞增殖和分化的影响。方法:通过双侧卵巢切除术建立骨质疏松动物模型。在培养的成骨细胞中,加入不同浓度的依普黄酮培养基(0M、10-9M、10-8M、10-7M和10-6M)继续培养72h。五组浓度依普黄酮,分别进行MTT、ALP和OC测定,得到作用最大的依普黄酮浓度。结果:依普黄酮能促进成骨细胞增殖、ALP表达和OC表达。并且随着依普黄酮浓度的增加,对成骨细胞增殖分化的促进作用先逐渐增强,然后减弱,其中10-8M浓度的依普黄酮的促进作用最大。结论:依普黄酮促进了骨质疏松大鼠颌骨成骨细胞的增殖分化能力,并且有一定的规律性,为依普黄酮在口腔医学中应用的深入研究提供基础。Objective:To investigate the effects of ipriflavone on proliferation and differentiation of the osteoblasts derived from the jaws of the adult osteoporotic rats.Methods:An osteoporotic animal model was established by performing a bilateral ovariectomy.After the osteoporotic osteoblasts were cultured,with different concentration ipriflavone medium(0M,10-9M,10-8M,10-7M and 10-6M)for 72h.MTT ALP and OC were used to determine the concentrations of ipriflavone in five groups.The ipriflavone concentration with the largest role was obtained.Results:The proliferation,ALP expression and OC expression of osteoblasts were promoted by ipriflavone.With the increasing concentration of ipriflavone,the promoting effect of ipriflavone on the proliferation and differentiation of osteoblasts first increased and then decreased,among which the promoting effect of 10-8M concentration of ipriflavone was the largest.Conclusion:Ipriflavone can promote the proliferation and differentiation of osteoblasts from the jaws of osteoporotic rats,and has a certain regularity,which provides a basis for the further study of the application of ipriflavone in stomatology.

关 键 词：成骨细胞 骨质疏松 颌骨 依普黄酮 

分 类 号：R787[医药卫生—口腔医学]