乳杆菌联合壳聚糖季铵盐对奶牛子宫内膜上皮细胞炎症的影响  

Effects of Lactobacillus and Chitosan Quaternary Ammonium Salt on Cow Endometrial Epithelial Cell Inflammation

作　　者：向炬富 刘红羽[1,2] 赵静 鄢冉[3] 王政业[4] 王军 吕文发 XIANG Jufu;LIU Hongyu;ZHAO Jing;YAN Ran;WANG Zhengye;WANG Jun;LYU Wenfa(Key Lab of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Jilin Changchun 130118,China;Joint Laboratory of Modern Agricultural Technology International Cooperation,Ministry of Education,Jilin Changchun 130118,China;College of Modern Agriculture,Changchun Polytechnic,Jilin Changchun 130118,China;Changchun Jiutai District Animal Health Supervision Institute,Jilin Changchun 130118,China)

机构地区：[1]吉林农业大学动物生产与产品质量安全教育部重点实验室,吉林长春130118 [2]现代农业技术教育部国际合作联合重点实验室,吉林长春130118 [3]长春职业技术学院现代农学院,吉林长春130118 [4]长春市九台区动物卫生监督所,吉林长春130118

出　　处：《中国畜牧杂志》2020年第8期116-121,共6页Chinese Journal of Animal Science

基　　金：国家重点研发计划资助项目(2018YDF0501600)。

摘　　要：本试验旨在探究魏斯氏乳杆菌(L. weissella)、唾液乳杆菌(L. salivarius)和约氏乳杆菌(L.johnsonii)联合壳聚糖季铵盐(CQN)对奶牛子宫内膜炎的影响。通过乳酸脱氢酶(LDH)释放检测法筛选致病性大肠杆菌(EPEC)最佳处理浓度和时间,建立奶牛子宫内膜上皮细胞炎症模型。诱导细胞炎症后,单独添加0.01 mg/mL CQN、108CFU/mL乳杆菌处理以及乳杆菌联合CQN处理,ELISA法和RT-qPCR法检测细胞因子IL-6、IL-8和IL-10的变化。结果表明:单独添加3株乳杆菌处理与单独添加CQN处理均降低了IL-6的蛋白和mRNA表达(P<0.05),但对IL-8的表达无显著影响;相比单独添加处理,约氏乳杆菌与CQN混合处理降低了IL-6和IL-8的表达(P<0.05),并恢复了IL-6/IL-10和IL8/IL-10的平衡;与CQN混合处理后,约氏乳杆菌对奶牛子宫内膜上皮细胞的黏附率提升(P<0.001)。结果提示,约氏乳杆菌联合CQN具有良好的抗炎潜力,可用于奶牛子宫内膜炎的治疗。The purpose of this study is to investigate the effects of three strains of L. weissella, L. salivarius, and L. johnsonii combined with chitosan quaternary ammonium salt(CQN) on cow endometritis. The concentration and time of cells treated by E. coli were screened by lactate dehydrogenase(LDH) release detection method, relying to which we established a cell inflammation model. After cell inflammation induced, the cells were treated by adding 0.01 mg/mL CQN alone, 108 CFU/mL Lactobacillus alone, and Lactobacillus combined with CQN. After that, expression of cytokines IL-6, IL-8 and IL-10 were detected by ELISA and RT-qPCR. The results showed that the addition of three strains of Lactobacillus alone and the addition of CQN alone significantly reduced the protein and mRNA expression of IL-6(P<0.05), but showed no significant effect on the expression of IL-8. However, compared with the single addition treatment, the mixed treatment of L. johnsonii and CQN significantly reduced the expression of IL-6 and IL-8(P<0.05) and restored the balance of IL-6/IL-10 and IL8/IL-10. At the same time, after mixed treatment with CQN, the adhesion rate of L. johnsonii to cow endometrial epithelial cells was significantly improved(P<0.001). The results suggested that alliance of L. johnsonii and CQN showed good anti-inflammatory potential, and it could be used in treat for cow endometritis.

关 键 词：乳杆菌 大肠杆菌 奶牛 子宫内膜炎 

分 类 号：S823.7[农业科学—畜牧学]