Acupuncture improved lipid metabolism by regulating intestinal absorption in mice  

作　　者：Jia Han Xin Guo Xiang-Jin Meng Jing Zhang Reimon Yamaguchi Yoshiharu Motoo Sohsuke Yamada 

机构地区：[1]Department of Pathology and Laboratory Medicine,Kanazawa Medical University,Ishikawa 920-0293,Japan [2]Department of Oncology,Kanazawa Medical University,Ishikawa 920-0293,Japan [3]Department of Pathology,Kanazawa Medical University Hospital,Ishikawa 920-0293,Japan [4]Department of Dermatology,Kanazawa Medical University,Ishikawa 920-0293,Japan

出　　处：《World Journal of Gastroenterology》2020年第34期5118-5129,共12页世界胃肠病学杂志（英文版）

基　　金：the Grants-in-Aid for Scientific Research,No.19K16783;the Ministry of Education,Culture,Sports,Science and Technology,Tokyo,Japan,No.20K07454 and No.20K17363;Grant for Promoted Research from Kanazawa Medical University,No.S2018-6.

摘　　要：BACKGROUND Non-alcoholic fatty liver disease(NAFLD),in which abnormal lipid metabolism plays an important role in disease progression,has become a pandemic.Abnormal lipid metabolism,for example an increased fat intake,has been thought to be an initial factor leading to NAFLD.The small intestine is the main site of dietary lipid absorption.A number of clinical trials have shown that acupuncture has positive effects in the regulation of lipid metabolism,which is closely associated with the progression of NAFLD.We therefore hypothesized that,acupuncture can improve the conditions of NAFLD by regulating intestinal absorption of lipid.AIM To study the role of acupuncture treatment in the improvement of metabolic syndrome secondary to NAFLD by mouse model.METHODS 8-wk-old male C57BL/6J mice were fed a methionine-and choline-deficient diet for 3 wk.Then,all mice were separated randomly into acupoints group(AG)or non-acupoints group(NG)with high fat diet feeding.Needling treatment was performed at Zu san li,Guan yuan and Yong quan acupoints as acupuncture treatment to AG mice while non-acupoints place to NG mice.Finally,mice were anesthetized with an injection of ketamine-medetomidine and euthanized by exsanguination.RESULTS An apparent improvement of obesity was found in AG mice after acupuncture treatment.In AG mice,the body weight was much lower(22.6±1.2 g vs 28.1±1.0 g,P<0.005)in comparison to NG mice.The length of small intestine in AG mice was significantly shorter(26.7±2.3 cm vs 32.7±2.7 cm,P<0.005).A large amount of chyme was observed in the lumen of the AG small intestine.The expression of microsomal triglyceride transfer protein,apolipoprotein B and apolipoprotein C2 was downregulated.Triacylglycerols(TGs),total cholesterol and nonesterified fatty acid(NEFA)levels of the small intestinal tissue were significantly higher in AG mice,but the serum TGs and NEFA levels were reduced in AG mice.CONCLUSION These results indicate that acupuncture at Zu san li,Guan yuan and Yong quan suppressed lipid absorption by do

关 键 词：ACUPUNCTURE Non-alcoholic fatty liver disease Small intestine Lipid metabolism APOLIPOPROTEINS 

分 类 号：R245[医药卫生—针灸推拿学]