苹果多酚-壳寡糖微胶囊的制备表征及释放特性  

Preparation,characterization and health benefit functions of unripe apple polyphenols-chitooligosaccharides microcapsule

作　　者：郑虎哲[1,2] Choi Jihye Seong Giun Chung Shinkyo Zheng Huzhe;Choi Jihye;Seong Giun;Chung Shinkyo(Department of Health Science,Jiangsu Food&Pharmaceutical Science College,Huai’an,223003,China;School of Food Science and Biotechnology,Kyungpook National University,Daegu,41566,Korea)

机构地区：[1]江苏食品药品职业技术学院健康学院,淮安223003 [2]庆北大学食品科学与工程学部,韩国大邱41566

出　　处：《农业工程学报》2020年第14期281-289,共9页Transactions of the Chinese Society of Agricultural Engineering

基　　金：Agriculture and fishery,Gyeongsangbukdo(No.GBTA 2016-04),Korea;Qinglan project(Su Teach 2019Ⅲ)in Jiangsu Province,China.

摘　　要：为了有效提高未熟苹果多酚(ApplePolyphenols,APP)和壳寡糖(Chitooligosaccharides,COS)多功能协调效应,该研究采用喷雾干燥法研制未成熟的苹果多酚-壳寡糖微胶囊(Apple Polyphenols-Chitooligosaccharides Microcapsule,APCM),并测定了APCM的微胶囊粒度和分布,以及结构表征,并评价了模拟胃肠道消化模型对总多酚(Total Phenolic Content,TPC)释放和健康益处功能的影响。激光粒度分析结果表明,APCM的平均粒径为32.98μm。跨度值最小为1.19,这意味着APCM比COS和APP更均匀、颗粒度更小。APP在1 237和1 194 cm^(-1)处观察到了清晰的峰形,在APCM中同一位置处未观察到。但是,APCM与APP具有相似的吸收带,这意味着APP与COS也可能通过范德华力和分子间氢键的方式形成APCM。模拟胃肠消化模型结果表明,APCM中多酚的释放发生在60min以内。在模拟胃液消化系统(SimulatedGastricFluid,SGF)处理中,APCM释放的TPC从25.6%到76.5%不等,而在模拟肠液消化系统(Simulated Intestinal Fluid,SIF)持续处理中,TPC释放量达到31.3%到97.6%。体外抗氧化活性和活性氧(Reactive Oxygen Species,ROS)清除活性结果表明,相比与APP和COS,APCM对抗氧化能力指数、清除DPPH自由基、铁离子还原抗氧化力、·OH清除活性、O_2^(·-)清除活性和H_2O_2清除活性表现出更出色的清除自由基活性。此外,与APP或COS相比,APCM不仅表现出更高的糖还原酶抑制活性(P<0.05),而且具有更好的血管紧张素I转换酶(Angiotensin I Converting Enzyme,ACE)抑制活性(P<0.05)。结果表明,APCM今后可在功能性食品或药物领域更大的研发潜力。In order to improve unripe apple polyphenols(APP)and chitooligosaccharides(COS)multifunctional effect,unripe apple polyphenols-chitooligosaccharides microcapsule(APCM)was prepared by spray-drying method.The microcapsule particle size,distribution,and the binding characters of APCM were detected.And the effects of simulated gastrointestinal digestion model on functional compounds release and health benefit functions were also evaluated.The results showed that the mean particle size of APCM was 32.98μm.In addition,APCM exhibited the smallest span value of 1.19,which meant APCM was more homogeneous than COS and APP.The results of binding characters showed APP had a sharper peak at 1237 and 1194 cm-1,which was not observed in APCM.However,APCM had similar absorption bands to the APP,which could be inferred that the combination of the APCM was due to hydrogen bonding,electrostatic,and van der Waals interactions between COS and APP.The simulated gastrointestinal digestion model results showed that the release of polyphenols from APCM occurred within 60 min.In simulated gastric fluid(SGF)treatment,the amount of total phenolic content(TPC)released from APCM ranged from 25.6%to 76.5%,and in the continuous incubation period in simulated intestinal fluid(SIF)treatment,the TPC released from 31.3%to 97.6%.The results of in vitro antioxidant and reactive oxygen species(ROS)scavenging activity indicated that APCM exhibited more excellent radical scavenging activity compared with that of APP and COS toward oxygen radical absorbance capacity(ORAC),1,1-diphenyl-2-picrylhydrazyl(DPPH),ferric reducing antioxidant power(FRAP),hydroxyl radical(•OH),superoxide anion radical(O2•-),and hydrogen peroxide radical(H2O2)scavenging activity,respectively.Furthermore,APCM exhibited not only higher aldose reductase inhibition activity(P<0.05),but also better Angiotensin I converting enzyme(ACE)inhibition activity(P<0.05)compared with APP and COS.The results suggest that APCM has greater potential for future research and development in the f

关 键 词：农产品 抗氧化 苹果多酚-壳寡糖微胶囊 模拟胃肠消化系统 释放率 醛糖还原酶 血管紧张素Ⅰ转换酶 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]