miR-145-3p在肝癌中的表达及其对肝癌细胞生长的调控作用  

Expression of miR-145-3p in hepatocellular cancer and its regulatory effect on growth of hepatocellular cancer cells

作　　者：叶小荣[1] 潘德标[2] 王理富[1] Xiao-Rong Ye;De-Biao Pan;Li-Fu Wang(Department of Trauma,Acute Abdomen and Hernia Surgery,Lishui City People’s Hospital,Lishui 323000,Zhejiang Province,China;Department of hepatobiliary Surgery,Lishui City People’s Hospital,Lishui 323000,Zhejiang Province,China)

机构地区：[1]丽水市人民医院创伤急腹症、疝与腹壁外科,浙江省丽水市323000 [2]丽水市人民医院肝胆外科,浙江省丽水市323000

出　　处：《世界华人消化杂志》2020年第17期827-833,共7页World Chinese Journal of Digestology

摘　　要：背景miR-145-3p在头颈癌、肺癌和胃癌等肿瘤可发挥抑癌的作用,而其在肝癌中的表达和作用并不清楚.目的探讨miR-145-3p在肝癌中的表达及其对肝癌细胞的增殖与凋亡的调控作用.方法用RT-qPCR法检测肝癌组织、癌旁组织、肝细胞株(Hep3B、Huh-7、HepG2和SMMC-7721)与正常肝细胞株L-02中miR-145-3p的表达水平;将miR-145-3p mimic或miR-145-3p inhibitor转入Hep3B细胞,用CCK-8法、流式细胞术、TUNEL法和Western blot法分别检测细胞活力、细胞周期、细胞凋亡和4型黏蛋白(mucin4,MUC4)表达.用荧光素酶报告基因实验鉴定miR-145-3p的靶基因.将pcDNA-MUC4转入已转染miR-145-3p mimic的细胞,用CCK-8法和TUNEL法分别检测细胞活力与细胞凋亡.结果miR-145-3p在肝癌组织和细胞中呈低表达.过表达miR-145-3p能抑制细胞增殖和G0/G1期到S期转换,促进凋亡和降低MUC4表达;而敲减miR-145-3p则作用相反.MUC4是miR-145-3p的靶基因.过表达MUC4能逆转miR-145-3p mimic对细胞存活的抑制作用.结论miR-145-3p在肝癌低表达,且其表达与T分期呈负相关.过表达miR-145-3p可抑制肝癌细胞存活与生长,而敲减miR-145-3p能促进肝癌细胞存活与生长.BACKGROUND miR-145-3p can play a tumor suppressive role in head and neck cancer,lung cancer,gastric cancer,and other tumors,while its expression and role in liver cancer are not clear.AIM To investigate the expression of miR-145-3p in hepatocellular cancer and its regulatory role in the proliferation and apoptosis of hepatocellular cancer cells.METHODS The expression levels of miR-145-3p in liver cancer tissues,paracancer tissues,liver cell lines(Hep3B,Huh-7,HepG2,and SMMC-7721),and the normal liver cell line L-02 were detected by RT-qPCR.After transfection of Hep3B cells with miR-145-3p mimic and miR-145-3p inhibitor,the cell viability,cell cycle,apoptosis,and the expression of mucin4(MUC4)were detected by CCK-8 assay,flow cytometry,TUNEL assay,and Western blot,respectively.The target gene of miR-145-3p was identified by luciferase reporter assay.pcDNA-MUC4 was transfected into miR-145-3p mimic-transfected cells,and cell viability and apoptosis were detected by CCK-8 and TUNEL assays,respectively.RESULTS miR-145-3p was lowly expressed in hepatocellular cancer tissues and cells.Overexpression of miR-145-3p inhibited cell proliferation and transition from G0/G1 phase to S phase,promoted apoptosis,and decreased MUC4 expression,while knockdown of miR-145-3p had the opposite effect.MUC4 was identified to be a target gene of miR-145-3p.Overexpression of MUC4 reversed the inhibitory effect of miR-145-3p mimic on cell survival.CONCLUSION miR-145-3p is lowly expressed in hepatocellular cancer,and its expression is negatively correlated with T stage.Overexpression of miR-145-3p can inhibit the survival and growth of hepatocellular cancer cells,while knockdown of miR-145-3p can promote the survival and growth of hepatocellular cancer cells.

关 键 词：miR-145-3p 肝癌 4型黏蛋白 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]