长链非编码RNA FAM83H-AS1对胃癌细胞周期、增殖和转移的影响及作用机制  

Effect and mechanism of long-chain non-coding RNA FAM83H-AS1 on cell cycle,proliferation and metastasis of gastric cancer

作　　者：王涛[1] 马牧原 叶晓锋[1] Wang Tao;Ma Muyuan;Ye Xiaofeng(The Second Dept of Oncology Surgery,General Hospital of Ningxia Medical University,Yinchuan 750001)

机构地区：[1]宁夏医科大学总医院肿瘤外二科,银川750001

出　　处：《安徽医科大学学报》2020年第9期1327-1331,共5页Acta Universitatis Medicinalis Anhui

基　　金：宁夏回族自治区卫生系统科研项目(编号:2015-NW-154)。

摘　　要：目的研究长链非编码RNA序列相似性家族83成员H反义RNA 1(lncRNA FAM83H-AS1)对胃癌细胞周期、增殖和转移的影响,并探讨其作用的可能机制。方法qRT-PCR检测FAM83H-AS1在胃癌细胞系MKN-45、SGC-7901、MGC-803和人永生化正常胃上皮细胞GES-1中的表达水平,选择表达最高的胃癌细胞系进行FAM83H-AS1小干扰核糖核酸(siRNA)感染,分为si-NC组和si-FAM83H-AS1组。qRT-PCR检测各组细胞中FAM83H-AS1的表达水平;流式细胞仪检测FAM83H-AS1对细胞周期的影响;MTS和平板克隆实验检测FAM83H-AS1对细胞增殖能力的影响;Tran-swell实验检测FAM83H-AS1对细胞转移能力的影响;West-ern blot检测FAM83H-AS1对cyclinD1和细胞周期蛋白依赖激酶4(CDK4)以及上皮间质转化过程(EMT)标志蛋白的表达影响。结果qRT-PCR检测结果显示FAM83H-AS1在胃癌细胞系的表达水平高于在人永生化正常胃上皮细胞GES-1中的表达(P<0.05),选择表达水平最高的胃癌细胞系MKN-45转染FAM83H-AS1 siRNA,qRT-PCR结果显示si-FAM83H-AS1组细胞中FAM83H-AS1的表达降低(P<0.05);si-FAM83H-AS1组MKN-45细胞增殖和转移能力下降,细胞中细胞周期蛋白cyclinD1和CDK4蛋白的表达降低,EMT标志蛋白E钙黏蛋白蛋白表达增加,N钙黏蛋白和波形蛋白蛋白表达降低。结论干扰FAM83H-AS1的表达抑制胃癌细胞增殖和转移能力。FAM83H-AS1可能是胃癌治疗的新型靶标。Objective To study the effect of long non-coding RNA family with sequence similarity 83 member H antisense RNA 1(lncRNA FAM83H-AS1)on cell cycle,proliferation and metastasis of gastric cancer cells,and explore its possible mechanisms.Methods qRT-PCR was used to detect the expression level of FAM83H-AS1 in gastric cancer cell lines MKN-45,SGC-7901,MGC-803 and human immortalized normal gastric epithelial cell line GES-1.The highest expression of gastric cancer cell line was selected for FAM83H-AS1 siRNA infection,which was divided into si-NC group and si-FAM83H-AS1 group.The expression of FAM83H-AS1 in each group was de-tected by qRT-PCR.The effect of FAM83H-AS1 on cell cycle was detected by flow cytometry.The effect of FAM83H-AS1 on cell proliferation was detected by MTS and plate cloning assay.The effect of FAM83H-AS1 on cell metastasis ability was detected by Transwell assay.Western blot was used to detect the effect of FAM83H-AS1 on the expression of cyclinD1 and cyclin dependent kinase 4(CDK4)and epithelial-mesenchymal transition(EMT)marker proteins.Results The results of qRT-PCR showed that the expression level of FAM83H-AS1 in gastric cancer cell lines was higher than that in human immortalized normal gastric epithelial cell line GES-1(P<0.05),and the gastric cancer cell line MKN-45 with the highest FAM83H-AS1 expression level was selected to transfect FAM83H-AS1 siRNA.qRT-PCR results showed that the expression of FAM83H-AS1 decreased in si-FAM83H-AS1 cells(P<0.05),the proliferation and metastasis ability and the expression of cyclinD1 and CDK4 proteins in si-FAM83H-AS1 group MKN-45 cells decreased,the expression of EMT marker protein E-cadherin pro-tein increased,and the expression of N-cadherin and Vinmentin protein decreased.Conclusion Interference with the expression of FAM83H-AS1 inhibits the proliferation and metastasis of gastric cancer cells.FAM83H-AS1 may be a novel target for the treatment of gastric cancer.

关 键 词：胃癌 lncRNA FAM83H-AS1反义RNA 1 细胞周期 增殖 转移 上皮间质转化 

分 类 号：R735.2[医药卫生—肿瘤]