继发性白血病患者T细胞亚群检测  

Detection of T cell subsets with secondary leukemia

作　　者：叶倩舲[1] 宋明珠 王会平[1] 肖浩 翟志敏[1] Ye Qianling;Song Mingzhu;Wang huiping(Dept of Hematology,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院血液内科,合肥230601

出　　处：《安徽医科大学学报》2020年第9期1469-1471,共3页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81602914)。

摘　　要：采用直接荧光标记法和多参数流式细胞术检测收集的继发性髓系白血病(S-AML)患者不同的T淋巴细胞亚群,例如总T淋巴细胞、辅助性T淋巴细胞、细胞毒性T淋巴细胞、调节性T细胞(Treg cells)和活化效应性T细胞(Teff cells)。Treg和Treg/Teff在S-AML、原发性髓系白血病(P-AML)和健康对照3组之间,差异有统计学意义(P<0.05)。急性髓系白血病患者免疫状态存在失衡,且S-AML患者免疫失衡较P-AML更为显著,这可能为将来预防和治疗S-AML提供新的思路。Direct fluorescence labeling and multiparameter flow cytometry were used to detect different T lympho-cyte subsets such as total T lymphocytes,helper T lymphocytes,cytotoxic T lymphocytes,regulatory T cells(Treg cells)and activated effector T cells(Teff cells)in patients with secondary myeloid leukemia retrospectively.By comparing secondary acute myeloid leukemia(S-AML),primary acute myeloid leukemia(P-AML)and healthy control group,the differences in Treg and Treg/Teff among the three groups were found to be statistically significant(P<0.05).There was an imbalance in the immune status of patients with acute myeloid leukemia.Moreover,the immune imbalance was even more pronounced in s-aml patients,which might provide new ideas for the prevention and treatment of S-AML in the future.

关 键 词：急性髓系白血病 T细胞 流式细胞术 治疗 

分 类 号：R733.712[医药卫生—肿瘤]