载血管生成抑制剂脂质微泡联合聚焦超声定向辐照治疗结肠癌肝转移的实验研究  

An experiment on the effect of endostar microbubble combined with focused ultrasound radiation on colon canear liver metastases

作　　者：王厚东[1] 杨关根[1] 张秀峰[1] 裘建明[1] 鲁振锋 俞艳艳[1] 沈忠[1] Wang Houdong;Yang Guangen;Zhang Xiufeng;Qiu Jianming;Lu Zhenfeng;Yu Yanyan;Shen Zhong(Department of Coloproctology,Hangzhou Third Hospital,Hangzhou 310009,China)

机构地区：[1]杭州市第三人民医院肛肠科,310009

出　　处：《中华普通外科杂志》2020年第8期644-648,共5页Chinese Journal of General Surgery

基　　金：浙江省卫生厅医药卫生平台重点资助计划项目(2013ZDA020);杭州市卫生科技计划一般项目(2013A27);杭州市科技局一般项目(20171226Y17)。

摘　　要：目的探讨载血管生成抑制剂脂质微泡联合聚焦超声定向辐照对结肠癌肝转移的治疗效果.方法29只结肠癌肝转移BaIb/c裸鼠随机分为4组,其中G1组8只裸鼠作为空白对照组;G2组7只裸鼠仅行超声辐照;G3组7只裸鼠采用超声辐照联合不负载药物的SonoVue裸微泡;G4组7只裸鼠采取超声辐照联合载血管内皮抑素Endstar处理.治疗结束后12d处死裸鼠,计数并称量各组原发肿瘤质量、肝转移瘤的数目及质量,用免疫组化方法检测CD34在肝转移瘤中的表达情况.结果G4组脾脏原位移植肿瘤质量为(0.79±0.49)g,低于G3组的(1.74±0.33)g、G2组的(2.60±0.60)g以及G1组的(2.67±0.61)g,差异有统计学意义(F=20.629,P<0.01).G4组肝脏转移瘤质量为(0.55±0.16)g,低于G3组的(0.95±0.27)g、G2组的(1.42±0.28)g及G1组的(1.47±0.22)g,差异有统计学意义(F=23.758,P<0.01).4组肝脏转移瘤结节数差异无统计学意义(F=0.167,P=0.918).G4组CD34灰度值为(8037±1708),低于G1组的(10931±1762)、G2组的(11570±2422)及G3组的(14978±1619),差异有统计学意义(F=15.779,P<0.01).结论载血管生成抑制剂脂质微泡联合聚焦超声定向辐照可以降低结肠癌肝转移瘤新生血管的生成,抑制原位肿瘤及肝转移瘤的生长.Objective To study the anti-tumor efficacy of endostar microbubble combined with focused ultrasound radiation in colon cancer liver metastases.Method 29 mice with colon cancer liver metastasis were randomly divided into four groups.Group 1(8 mice),as the control group.Group 2(7 mice)were treated only with ultrasonic radiation.Group 3(7 mice)treated with the ultrasonic radiation combined with SonoVue microbubbles without carrying any medicine.Group 4(7 mice),treated with the ultrasonic radiation combined with microbubbles carrying endostar.The mice were sacrifced and the tumor specimens were weighted on the 12 days after ultrasound radiation.Immunohistochemistry was used to assess CD34 expression within the metastatic tumor.Results The tumor weight in group4(0.79±0.49)g was significantly lower than that in group1(2.67±0.61)g,group2(2.60±0.60)g and group3(1.74±0.33)g(F=20.629,P<0.01).The liver metastatic tumor weight in group 4(0.55±0.16)g was much lower than that in group1(1.47±0.22)g,group 2(1.42±0.28)g and group3(0.95±0.27)g(F=23.758,P<0.01).There was no obvious difference among the four groups in the number of nodules of metastatic tumor in liver(F=0.167,P=0.918).The level of CD34 in group4 were(8037±1708),significantly lower than that in any other group,(F=15.779,P<0.01)。Conclusion Endostar microbubble combined with focused ultrasound radiation decreases tumor angiogenesis in liver metastasis,and inhibits the growth of both primary and metastatic tumor.

关 键 词：结肠肿瘤 肿瘤转移 超声疗法 血管内皮抑素 

分 类 号：R735.35[医药卫生—肿瘤]