长链非编码RNA FEZF1-AS1在喉癌中的表达及意义  

Expression and clinical significance of long non-coding RNA FEZF1-AS1 in laryngeal cancer

作　　者：曲丹菊[1] 张超[1] 苏吉利[1] QU Dan-ju;ZHANG Chao;SU Ji-li(Department of Otorhinolaryngology,the First Afiliated Hospital of Henan University of Science And Technology,Luuoyang 471000,Henan,China)

机构地区：[1]河南科技大学第一附属医院(河南科技大学临床医学院)耳鼻咽喉科,河南洛阳471000

出　　处：《广东医学》2020年第15期1562-1566,共5页Guangdong Medical Journal

基　　金：河南省医学科技攻关计划项目(201504020)。

摘　　要：目的探究长链非编码RNA FEZ家族锌指1反义RNA1(Lnc RNA FEZF1-AS1)在喉癌中的表达及临床意义.方法选取术前未经治疗的喉癌患者78例组织标本作为研究对象,同期取喉癌旁黏膜组织78例作为对照组,采用实时荧光定量PCR(qRT-PCR)法检测FEZF1-AS1表达水平;分析FEZF1-AS1表达水平与喉癌患者临床病理特征及喉癌患者近3年生存情况;COX回归分析喉癌患者预后的危险因素.结果与喉癌旁黏膜组织相比,喉癌组织FEZF1-AS1表达水平较高(P<0.05);FEZF1-AS1表达水平与性别、年龄、喉癌原发部位、病理学分级无关(P>0.05),与临床分期、T分级、淋巴结转移有关(P<0.05);生存分析发现,FEZF1-AS1高表达喉癌患者中位生存时间为13.60个月,显著短于FEZF1-AS1低表达患者中位生存时间(23.44个月),差异有统计学意义(P<0.05);COX回归分析结果,有淋巴结转移、FEZF1-AS1高表达是喉癌患者不良预后的独立危险因素(HR=1.589,95%CI为1.362~1.854;HR=2.375,95%CI为1.367~4.127,P<0.05).结论FEZF1-AS1在喉癌组织中高表达,是喉癌患者预后不良危险因素,可能成为喉癌患者预后不良的生物监测指标.Objective To investigate the expression and clinical significance of FEZ family zinc finger 1 antisense RNA1(lncrnFEZF1-AS1) in laryngeal cancer. Methods Tissue specimens of 78 cases of untreated laryngeal cancer were selected as the study objects, and tissue specimens of 78 cases of laryngeal mucosa were taken as the control group. The expression of FEZF1-AS1 was assessed by real-time fluorescent quantitative PCR(qRT-PCR). The expression level of FEZF1-AS1, the clinicopathological characteristics of laryngeal cancer patients and the survival of laryngeal cancer patients in the recent 3 years were analyzed. COX regression was used to analyze the risk factors of prognosis in patients with laryngeal cancer. Results The expression level of FEZF1-AS1 in laryngeal cancer was significantly higher than that in adjacent mucosa(P<0.05);the expression level of FEZF1-AS1 was not correlated to gender, age, primary site of laryngeal cancer or pathological grade(P>0.05), but significantly correlated to clinical stage, T grade and lymph node metastasis(P<0.05). Survival analysis showed that the median survival of patients with high expression of FEZF1-AS1 was 13.60 months, which was significantly shorter than 23.44 months of patients with low expression of FEZF1-AS1(P<0.05). COX regression analysis showed that lymph node metastasis and high expression of FEZF1-AS1 were independent risk factors for poor prognosis of patients with laryngeal cancer(HR=1.589, 95%CI: 1.362-1.854;HR=2.375, 95%CI: 1.367-4.127, P<0.05). Conclusion The FEZF1-AS1 is highly expressed in laryngeal cancer, which is a risk factor for poor prognosis of patients with laryngeal cancer, and may become a biological monitoring index for poor prognosis of patients with laryngeal cancer.

关 键 词：喉癌 长链非编码RNA FEZ家族锌指1反义RNA1 临床意义 预后 

分 类 号：R739.65[医药卫生—肿瘤] R34[医药卫生—临床医学]