基于网络药理学的丹参治疗肝硬化的机制研究  

Exploration on mechanisms of Salvia miltiorrhiza in treating cirrhosis based on network pharmacology

作　　者：陈红旋 胡敬宝 张琳琳 陈柏书[2] 鲁艳平[2] 杨从意[2] 陈慧基 刘茜茜 李舒 尹建平[2] CHEN Hong-xuan;HUJing-bao;ZHANG Lin-lin;CHEN Bai-shu;LU Yan-ping;YANG Cong-yi;CHEN Hui-ji;LIU Qian-qian;LI Shu;YIN Jian-ping(Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China;Shenzhen Baoan Hospital of Traditional Chinese Medicine Afiliated to Guangzhou University of Traditional Chinese Medicine,Shenzhen 518101,China;The Second Clinical College of Guangzhou University of TCM,Guangzhou 510405,China)

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学附属深圳市宝安中医院(集团),广东深圳518101 [3]广州中医药大学第二临床医学院,广东广州510405

出　　处：《中草药》2020年第15期3968-3977,共10页Chinese Traditional and Herbal Drugs

摘　　要：目的利用网络药理学的方法,探索丹参治疗肝硬化的可能作用机制。方法通过TCMSP数据库得出丹参的活性成分,再利用GeneCards、OMIM数据库以及DRAR-CPI服务器筛选丹参的活性成分治疗肝硬化的潜在作用靶点,进而用Cytoscape3.6.0软件构建丹参的化合物-靶点网络图。通过STRING数据库和Cytoscape3.6.0软件的Generatestylefrom statistics工具,筛选并构建疾病靶点相互作用网络。采用Systems Dock Web Site网络服务器与丹参的活性成分进行分子对接。利用DAVID数据库对丹参的作用靶点进行GO分类富集分析和KEGG通路富集分析。结果选择口服生物利用度(OB)≥30%和类药性(DL)≥0.18作为化合物分子的筛选条件,从丹参中筛选出活性成分65个,作用靶标75个。丹参治疗肝硬化主要涉及MAPK、Toll-like receptor,Gap junction、PI3K/Akt、Natural killer cell mediated cytotoxicity等信号通路。结论应用网络药理学的方法预测出丹参治疗肝硬化的可能作用机制,为其进一步研究提供新的思路与线索。Objective Network pharmacology method was adopted in this study to explore the active compounds and mechanism of Salvia miltiorrhiza for cirrhosis.Methods TCMSP database was utilized to obtain the active components of S.miltiorrhiza.Through GeneCards,OMIM and DRAR-CPI,the potential targets of S.miltiorrhiza for the treatment of cirrhosis were screened.Cytoscape 3.6.0 software was established to construct the active components-targets network of S.miltiorrhiza.STRING database and Generate style from statistics of Cytoscape 3.6.0 software were conducted to draw a graph of protein interaction network.Molecular docking was carried out through Systems Dock Web Site with the active components of S.miltiorrhiza.The GO classified enrichment analysis and the KEGG pathway enrichment analysis were performed by using DAVID database.Results Selecting the OB≥30%and DL≥0.18 as filter condition,65 active components and 75 targets of S.miltiorrhiza were involved.S.miltiorrhiza exerted its effects on treating cirrhosis mainly by regulating signaling pathways including MAPK,Toll-like receptor,Gap junction,PI3K/AKT,Natural killer cell mediated cytotoxicity signaling pathway and so on.Conclusion This study preliminarily predicted the major targets and pathways of S.miltiorrhiza acting on cirrhosis,which provided new ideas and clues for its further research.

关 键 词：丹参 肝硬化 网络药理学 活性成分 分子对接 

分 类 号：R285.5[医药卫生—中药学]