单核钌配合物的合成、表征及抗肿瘤活性研究  

Synthesis,Characterization and Antitumor Activity of Mononuclear Ruthenium(Ⅱ)Complexes

作　　者：张燕[1] 贾士芳[1] ZHANG Yan;JIA Shifang(School of Chemical and Biological Engineering, Taiyuan University of Science and Technology, Taiyuan 030021, China)

机构地区：[1]太原科技大学化学与生物工程学院,太原030021

出　　处：《太原理工大学学报》2020年第5期690-696,共7页Journal of Taiyuan University of Technology

基　　金：山西省高等学校科技创新项目(2020L0334);太原科技大学博士启动基金资助项目(20172005)。

摘　　要：合成了两个含有硫醚配体的单核钌配合物Ru1和Ru2,通过元素分析、红外光谱、电喷雾质谱和核磁氢谱对其进行了表征,并做了紫外-可见吸收光谱及电化学测试。通过体外细胞毒性实验研究了Ru1和Ru2对宫颈癌细胞(Hela)、胃癌细胞(BGC823)和乳腺癌细胞(MCF-7)三种肿瘤细胞的细胞毒性。结果表明:Ru1的抗肿瘤活性比Ru2强,而Ru1和Ru2均对MCF-7最具有选择性,初步说明Ru1和Ru2有作为抗癌药物的潜力。最后通过荧光染色和流式细胞仪观察Ru1对MCF-7细胞凋亡情况。Two mononuclear ruthenium complexes were synthesized and characterized by elemental analysis,infrared spectroscopy,1H NMR,and electrospray ionization mass spectrometry.Their photophysical and electrochemical properties were also studied.The cytotoxicity to cervical cancer(Hela),gastric cancer(BGC823),and human breast cancer(MCF-7)of the two complexes in vitro was evaluated by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.It is worth noting that Ru1 and Ru2 showed excellent antitumour effects in a cellular study for MCF-7 in vitro.However,Ru1 exhibited higher cytotoxicity to any cancer cells than Ru2.The results reveal that Ru1 and Ru2 have obvious selectivity and might be a potential anticancer agent to improve the efficacy of common anticancer therapies.The mode of cell apoptosis was assessed by Hoechst staining and flow cytometry assay.

关 键 词：钌配合物 光谱性质 电化学性质 抗肿瘤活性 细胞凋亡 

分 类 号：O614.121[理学—无机化学]