CD155蛋白在胰腺癌组织和细胞系中的表达及其临床意义  

Expression of CD155 protein in pancreatic cancer tissues and cell lines and its clinical significance

作　　者：周海波 陈炯 邬高华 胡丕波 何俊飞 Zhou Haibo;Chen Jiong;Wu Gaohua(Dept of General Surgey,The Affiliated Prouincial Hospital of Anhui Medicine University,Hefei 230001;Laboratory of Hepatopancreatobiliary Surgery,The Affiliated Prouincial Hospital of Anhui Medicine University,Hefei 230001)

机构地区：[1]安徽医科大学附属省立医院普外科,合肥230001 [2]安徽医科大学附属省立医院肝胆胰外科实验室,合肥230001

出　　处：《安徽医科大学学报》2020年第8期1294-1298,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81071985);安徽省国际科技合作项目(编号:1008070038)。

摘　　要：目的研究CD155蛋白在胰腺导管腺癌组织和癌旁正常组织以及在胰腺癌细胞系中的表达及其临床意义。方法采用免疫组织化学方法检测92例胰腺导管腺癌配对的石蜡包埋组织和癌旁正常组织中CD155蛋白的表达情况,并研究CD155与胰腺导管腺癌患者临床病理参数之间的关系,同时利用Western blot和qRT-PCR法检测正常胰腺细胞(HPDE6-C7)和3株胰腺癌细胞(Panc-1、BxPC-3、AsPC-1)中CD155的表达情况。结果免疫组化结果显示,CD155主要表达于胰腺导管腺癌组织的细胞膜上,CD155在胰腺导管腺癌组织中阳性表达率为65.22%(60/92),而在癌旁正常组织中阳性表达率为38.04%(35/92),两组阳性表达的差异有统计学意义(χ~2=13.60,P<0.01);CD155表达与分化程度(P<0.001)、神经浸润(P<0.001)、淋巴结转移(P=0.001)均有相关性;Western blot及qRT-PCR结果显示,3株胰腺癌细胞中CD155蛋白及mRNA的表达量均高于正常胰腺细胞,其差异均有统计学意义(P<0.01)。结论CD155的表达与胰腺导管腺癌的分化程度、神经浸润及淋巴结转移均相关,CD155的检测有助于胰腺导管腺癌患者病情的严重程度的评估,同时鉴于CD155在胰腺癌细胞中的高表达,利用CD155与免疫细胞活化性受体DNAM-1结合,启动免疫细胞对胰腺癌的活化杀伤作用,为胰腺癌的免疫治疗提供新思路。Objective To study the expression and clinical significance of CD155 protein in pancreatic ductal adenocarcinoma tissues and adjacent normal tissues,as well as in pancreatic cancer cell lines.Methods Immunohistochemistry was used to detect the expression of CD155 in paraffin-embedded tissues and non-carcinoma adjacent tissues from 92 cases of pathologically confirmed pancreatic ductal adenocarcinoma.And the relationship between CD155 and clinical pathological parameters was analyzed.Western blot and qRT-PCR methods were also used to detect the expression of CD155 in normal pancreatic cells(HPDE6-C7)and three pancreatic cancer cells(Panc-1,BxPC-3,AsPC-1).Results The results of immunohistochemistry showed that CD155 was mainly expressed on the cell membrane of pancreatic ductal adenocarcinoma tissues.The positive expression rate of CD155 in pancreatic ductal adenocarcinoma tissues was 65.22%(60/92),while the positive expression rate in normal tissues adjacent to the cancer was 38.04%(35/92),the difference in positive expression between the two groups was statistically significant(χ~2=13.60,P<0.01);The expression of CD155 was ralated to the degree of pancreatic ductal adenocarcinoma differentiation(P<0.001),neural infiltration(P<0.001),and lymph node metastasis(P=0.001);Westen blot and qRT-PCR results showed that the expression levels of CD155 protein and mRNA in three pancreatic cancer cells were higher than those in normal pancreatic cells,and the differences were statistically significant compared with normal pancreatic cells(P<0.01).Conclusion The expression of CD155 is correlated with the degree of pancreatic ductal adenocarcinoma differentiation,nerve invasion and lymph node metastasis.The detection of CD155 is used to evaluate the severity of pancreatic ductal adenocarcinoma patients.At the same time,CD155 is highly expressed in pancreatic cancer cells.It may be possible to use CD155 to bind to the immune cell activating receptor DNAM-1 to initiate the activation and killing effect of immune cells on pancrea

关 键 词：CD155 胰腺癌 免疫组织化学 Western blot QRT-PCR 

分 类 号：R735.9[医药卫生—肿瘤]