佳乐麝香对斑马鱼胚胎甲状腺激素的影响  

Effect of Galaxolide on Thyroid Hormones in Zebrafish Embryos

作　　者：李明 尹晓宇 陈浩 王欢 宋磊 董文静 赵宝全 于永利 董武[1] 杨景峰 Li Ming;Yin Xiaoyu;Chen Hao;Wang Huan;Song Lei;Dong Wenjing;Zhao Baoquan;Yu Yongli;Dong Wu;Yang Jingfeng(Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology,Collage of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028000,China;State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;College of Life Science and Food,Inner Mongolia University for Nationalities,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学动物科学技术学院,内蒙古自治区毒物监控及毒理学重点实验室,通辽028000 [2]军事科学院军事医学研究院毒物药物研究所,抗毒药物与毒理学国家重点实验室,北京100850 [3]内蒙古民族大学生命科学与食品学院,通辽028000

出　　处：《生态毒理学报》2020年第3期81-89,共9页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金资助项目(21567019,81360508);内蒙古自治区自然科学基金资助项目(2018MS08033,2020MS08103);内蒙古自治区高等学校科学研究项目(NJZC17203);内蒙古草原英才2020年度滚动支持项目(董武);内蒙古民族大学科学研究基金资助项目(NMDGP1505);内蒙古自治区毒物监控及毒理学重点实验室开放项目(MDK2019074,MDK2019076);蒙药研发国家地方联合工程研究中心开放基金资助项目(MDK2019051)。

摘　　要：佳乐麝香(galaxolide,HHCB)作为主要的多环麝香之一,被广泛应用于药品和个人护理品(pharmaceuticals and personal care products,PPCPs)中,残留在环境中造成污染,并在生物体内富集,对水生生物及人类健康造成潜在威胁。为探究环境浓度下HHCB的环境危害,选用斑马鱼(Danio rerio)作为模式动物,监测了HHCB暴露下其死亡率、孵化率、心率、心囊面积、甲状腺素含量和甲状腺激素关联基因表达量的变化,并探究相关毒理机制。研究结果表明,HHCB可导致斑马鱼胚胎死亡率增加、心率降低、孵化率下降以及甲状腺素关联基因Dio2、Thrα和Thrβ的显著变化。0.005 mmol·L^-1 HHCB可引起总三碘甲状腺原氨酸(TT3)升高和总四碘甲状腺原氨酸(TT4)的显著降低。通过以上研究可知,HHCB可造成斑马鱼胚胎甲状腺激素分泌和调节的紊乱。As one of the main polycyclic musk,galaxolide(HHCB)is widely used in pharmaceuticals and personal care products(PPCPs),the residues cause pollution in the environment and are enriched in organisms,posing potential threats to aquatic organisms and human health.In order to investigate the toxic effects of HHCB at environmental concentrations,zebrafish(Danio rerio)was used as an animal model to monitor the changes in mortality,hatchability,heart rate,heart sac area,thyroxine content,and thyroid-related gene expression after HHCB treatment,and explore the toxicological mechanism.The results showed that the exposure of HHCB(0.005 mmol·L^-1)caused increased zebrafish embryos mortality,decreased heart rate,lowered hatching rate and produced significant changes in thyroid hormone-related genes Dio2,Thrα,and Thrβ,and significantly decreased thyroid hormone triiodothyro-nine(TT3)and increased thyroxine(TT4)in zebrafish embryos.The above studies indicated that exposure of HHCB can cause disorders in thyroid hormone secretion and regulation in developmental zebrafish embryos.

关 键 词：佳乐麝香 斑马鱼胚胎 急性暴露 发育毒性 甲状腺激素 

分 类 号：X171.5[环境科学与工程—环境科学]