降解霉变玉米中黄曲霉毒素B1的复合益生菌研制  

Preparation of compound beneficial microbes for degrading aflatoxin B1 in moldy corn

作　　者：赵闪闪 周璞 刘硕 常娟[1] 王平[1] 尹清强[1] 刘超齐 朱群 Zhao Shanshan;Zhou Pu;Liu Shuo;Chang Juan;Wang Ping;Yin Qingqiang;Liu Chaoqi;Zhu Qun

机构地区：[1]河南农业大学牧医工程学院,河南郑州450046 [2]舞钢市畜产品质量检测中心,河南舞钢462599 [3]叶县动物疫病预防控制中心,河南叶县467200 [4]河南德邻生物制品有限公司,河南新乡453000

出　　处：《饲料工业》2020年第17期53-56,共4页Feed Industry

基　　金：河南省自然科学基金[182300410029];新乡市重大科技专项[ZD19005]。

摘　　要：为了降解霉变玉米中黄曲霉毒素B1(AFB1),该研究选用具有降解AFB1功能的产朊假丝酵母、枯草芽孢杆菌、干酪乳杆菌、丁酸梭菌,利用响应面回归设计获得最佳的降解AFB1的益生菌组合。结果表明,当产朊假丝酵母、枯草芽孢杆菌、干酪乳杆菌、丁酸梭菌的活菌数为1×10^6、1×10^6、1×10^4、1×10^6CFU/g时,在37℃发酵霉变玉米4 d后,使AFB1的降解率达到59.18%(P<0.05),该研究对提高霉变饲料的饲用价值具有重要意义。In order to degrade aflatoxin B1(AFB1)in moldy corn,Candida utilis,Bacillus subtilis,Lactobacillus casei and Clostridium butyrate with AFB1-degradation function were selected in this study.The compound beneficial microbes for AFB1 degradation were prepared by response surface regression design.The result showed that AFB1-degradation rate reached 59.18%(P<0.05)when the visible counts of Candida utilis,Bacillus subtilis,Lactobacillus casei and Clostridium butyrate were 1×10^6,1×10^6,1×10^4 and 1×10^6 CFU/g respectively after fermentation at 37℃for 4 d.This result had important roles in increasing the feeding value of moldy feedstuffs.

关 键 词：益生菌 黄曲霉毒素B1 霉变玉米 霉菌毒素降解 微生物发酵 

分 类 号：S816.3[农业科学—饲料科学]