17β-雌二醇对N-甲基-N-亚硝脲诱导大鼠视网膜损伤的保护效应  

Protective effect of 17β-estradiol against N-methyl-N-nitrosourea-induced retinal damage in rats

作　　者：朱春晖 朱若晨[1] 熊业城 陈涛 俞小瑞 ZHU Chunhui;ZHU Ruochen;XIONG Yecheng;CHEN Tao;YU Xiaorui(Department of Biochemistry and Molecular Bio-logy,School of Basic Medical Sciences,Xi’an Jiaotong University Health Science Center,Xi’an Jiaotong University,Xi’an 710006,China;Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases;Department of Periodontology,College of Stomatology,Xi’an Jiaotong University;Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education,Xi’an Jiaotong University)

机构地区：[1]西安交通大学医学部基础医学院生物化学与分子生物学系,西安710061 [2]陕西省牙颌疾病临床研究中心 [3]西安交通大学口腔医院牙周科,西安710061 [4]西安交通大学医学部环境与基因相关疾病教育部重点实验室,西安710061

出　　处：《山西医科大学学报》2020年第8期834-840,共7页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81870676,81901019)。

摘　　要：目的评估17β-雌二醇(βE2)对N-甲基-N-亚硝脲(MNU)诱导的视网膜感光细胞凋亡的神经保护作用。方法35日龄雌性SD大鼠随机分为对照(control)和MNU组,每组12只。为进一步观察βE2对MNU诱导大鼠视网膜损伤的保护作用,大鼠随机分为生理盐水(saline)-MNU和βE2-MNU组,每组12只。给药1 d后4组各取6只大鼠采用TUNEL检测视网膜凋亡细胞,给药7 d后各取6只大鼠通过HE染色和ERG检测视网膜形态结构和功能。最后,为了揭示βE2对视网膜的保护作用和抗氧化的关系,大鼠随机分为saline组,βE2组,saline-MNU组和βE2-MNU组,每组6只;各组药物处理后1 d使用试剂盒检测视网膜组织丙二醛(MDA)含量、抑制羟自由基能力及总抗氧化能力(T-AOC)。大鼠行卵巢摘除术后2周MNU组经腹腔注射45 mg/kg MNU;saline组和βE2组分别经玻璃体腔注射4μl的saline或βE2(100μmol/L);saline-MNU组和βE2-MNU组经腹腔注射45 mg/kg MNU后立即经玻璃体腔分别注射4μl的saline或100μmol/LβE2;未做处理的同天数大鼠设置为control。结果与control组相比,MNU组给药后1 d感光细胞凋亡数量显著增加,给药后7 d视网膜结构紊乱、功能下降。与saline-MNU组相比,βE2-MNU组大鼠给药后1 d感光细胞凋亡数量显著减少,给药后7 d视网膜功能增强,结构恢复。与saline组相比,saline-MNU组大鼠MDA升高,抑制羟自由基能力和T-AOC水平降低,差异有统计学意义(P<0.05)。与saline-MNU组相比,βE2-MNU组大鼠MDA降低,抑制羟自由基能力和T-AOC水平升高,差异有统计学意义(P<0.05)。结论βE2可以恢复MNU诱导的大鼠视网膜的损伤,其机制可能与抑制氧化应激水平有关。Objective To evaluate the neuroprotective effect of 17β-estradiol(βE2)against N-methyl-N-nitrosourea(MNU)-induced apoptosis of retinal photoreceptor cells.Methods Twnenty-four 35-day-old SD rats were randomly divided into control group and MNU group(12 in each group).To further determine the protective effect ofβE2 against MNU-induced retinal damage in rats,rats were randomly divided into saline-MNU group andβE2-MNU group(12 in each group).Six rats were chosen to detect the apoptosis in above four groups one day after treatment by TUNEL,and the other 6 rats were chosen to observe the retinal morphology and function by HE and ERG seven days after treatment.Finally,in order to reveal the relationship between the protective effect ofβE2 on the retina and its anti-oxidation,the rats were randomly divided into saline group,βE2 group,saline-MNU group andβE2-MNU group(6 in each group).Then the kits were utilized to measure the content of malondialdehyde(MDA),the ability to inhibit hydroxyl radicals and the total antioxidant capacity(T-AOC)in retinal tissue one day after treatment.All rats underwent ovarian removal.At week 2 after surgery,the rats in MNU group were intraperitoneally injected with 45 mg/kg MNU,rats in saline group andβE2 group were injected intravitreally with 4μl saline orβE2(100μmol/L),respectively,and rats in saline-MNU group andβE2-MNU group were intraperitoneally injected with 45 mg/kg MNU and then immediately intravitreally injected with 4μl saline orβE2(100μmol/L),respectively.Rats without treatment were set as control at the same time.Results Compared with control group,the number of apoptotic photoreceptor cell was increased significantly at day 1 after intraperitoneal injection in MNU group.Compared with control group,the retinal structure was disordered and the function was decreased at day 7 after intraperitoneal injection in MNU group.Compared with saline-MNU group,the number of photoreceptor cell apoptosis was significantly reduced at day 7 after drug treatment inβE2-MNU grou

关 键 词：17Β-雌二醇 N-甲基-N-亚硝脲 视网膜损伤 

分 类 号：R774.1[医药卫生—眼科]