HSPE1-MOB4在肿瘤细胞中的表达及其新剪接形式的鉴定  

The expression of HSPE1-MOB4 in tumor cells and identification of a novel splicing form

作　　者：刘小艳 黄浩 鲁梅芳 李洪 吕正梅[1] 蔡春林 Liu Xiaoyan;Huang Hao;Lu Meifang(Dept of Histology and Embryology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032;Dept of Reproductive Medicine,Huangshi Central Hospital,Huangshi 435000)

机构地区：[1]安徽医科大学基础医学院组胚教研室,合肥230032 [2]黄石市中心医院生殖科,黄石435000

出　　处：《安徽医科大学学报》2020年第7期992-996,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81572364)。

摘　　要：目的探究热休克蛋白家族成员1(HSPE1)和鼠类人α-趋化因子类似物(MOB4)形成的融合蛋白HSPE1-MOB4在不同细胞中的表达。方法采用逆转录聚合酶链式反应(RT-PCR)检测融合蛋白HSPE1-MOB4在不同细胞中的表达;Sanger基因测序探究融合蛋白的不同亚型;免疫荧光技术和激光共聚焦显微成像检测蛋白表达位置;免疫印迹法检测融合蛋白HSPE1-MOB4在正常细胞及肿瘤细胞中的表达。结果融合蛋白HSPE1-MOB4在正常细胞及肿瘤细胞中均有表达;基因测序结果显示融合蛋白HSPE1-MOB4有两种不同亚型,且两种亚型的表达位置相同;相较于正常细胞,在肿瘤细胞中融合蛋白的表达量更高。结论融合蛋白HSPE1-MOB4有两个亚型,已报道的亚型在肿瘤细胞中表达量较正常细胞高。这可能为寻找新的肿瘤生物标志物提供新的思路。Objective To investigate the expression of fusion protein HSPE1-MOB4 in heat shock protein family member 1(HSPE1)and murine human-like alpha-chemokine analog1(MOB4)in different cells.Methods Reverse transcription polymerase-chain reaction(RT-PCR)was used to detect the expression of fusion protein HSPE1-MOB4 in different cells.Sanger gene sequencing was used to explore different subtypes of fusion protein.Protein position in cells was detected by immunofluorescence technique and laser confocal microscopy.The expression of fusion protein HSPE1-MOB4 in normal cells and tumor cells was detected by immunoblotting.Results The fusion protein HSPE1-MOB4 was expressed in normal cells and tumor cells.The results of gene sequencing showed that the fusion protein HSPE1-MOB4 had two different subtypes,and the expression positions of the two subtypes were the same.Compared with normal cells,the amount of fusion protein expressed in tumor cells was higher.Conclusion The fusion protein HSPE1-MOB4 has two subtypes and the reported one is highly expressed in tumor cells.This may provide new ideas for finding new tumor biomarkers.

关 键 词：融合蛋白HSPE1-MOB4 Sanger基因测序 免疫荧光技术 

分 类 号：R735.2[医药卫生—肿瘤]