右美托咪定对人肝癌Huh-7细胞增殖和迁移的影响  

Effect of dexmedetomidine on proliferation and migration of Huh-7 human hepatocellular carcinoma cells

作　　者：虞乐 黄春霞 胡军[1,2] 周大臣 张野[1,2] Yu Le;Huang Chunxia;Hu Jun(XKey Laboratory of Anesthesiology and Perioperative Medicine of Anhui Higher Education Institutes,Hefei 230601;Dept of Anesthesiology and Perioperative Medicine,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]麻醉与围术期医学安徽普通高校重点实验室,合肥230601 [2]安徽医科大学第二附属医院麻醉与围术期医学科,合肥230601 [3]安徽医科大学第二附属医院肝胆外科,合肥230601

出　　处：《安徽医科大学学报》2020年第7期1019-1023,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81471145、81801050);安徽医科大学基础与临床提升研究计划(编号:2019xkjT026)。

摘　　要：目的探讨右美托咪定对人肝癌Huh-7细胞增殖和迁移的影响及其潜在机制。方法实验分成细胞和动物两部分。首先,将人肝癌Huh-7细胞随机分为6组:对照组(CON组)、0.1 nmol/L右美托咪定组(D1组)、1 nmol/L右美托咪定组(D2组)、10 nmol/L右美托咪定组(D3组)、氧糖剥夺/复氧复糖组(OGD/R组)、右美托咪定+氧糖剥夺/复氧复糖组(D+OGD/R组)。采用MTT比色法检测细胞活性,Western blot测定SIRT1的蛋白表达量;采用Transwell法检测细胞迁移能力。其次,将SPF级BALB/c-nu雄鼠6只,随机分为对照组(CON组)和右美托咪定组(DEX组)。皮下种植Huh-7细胞,DEX组裸鼠注射右美托咪定(5 mg/kg),CON组注射相应体积的生理盐水,连续6 d,种瘤30 d后处死裸鼠,取出瘤体,计算肿瘤体积。结果右美托咪定可以促进Huh-7细胞的细胞活力及迁移能力,减轻因OGD/R造成的损伤,同时增加SIRT1的表达。而右美托咪定对裸鼠肿瘤的体积无明显影响。结论右美托咪定可以增强人肝癌Huh-7细胞株的增殖和迁移能力,这种作用可能是通过上调SIRT1表达来完成。Objective To study whether dexmedetomidine could affect the proliferation and migration of Huh-7 human hepatocellular carcinoma cells and to investigate the potential molecular mechanism.Methods The experiment was divided into two parts:cells and animals.Firstly,Huh-7 cells were randomly classified into six groups:normal control(group CON),dexmedetomidine 0.1 nmol/L(group D1),dexmedetomidine 1 nmol/L(group D2),dexmedetomidine 10 nmol/L(group D3),group OGD/R,OGD/R with dexmedetomidine 0.1 nmol/L(group D+OGD/R).Cells proliferation was measured by MTT assay.SIRT1 protein expression was assessed by Western blot.Transwell migration assay was used to detect the migration of Huh-7 cells.Secondly,the mouse xenograft model of hepatocellular carcinoma was established,and then randomly divided into 2 groups:group DEX and group CON.Normal saline or dexmedetomidine(0.5 mg/kg)was daily injected(s.c.)to the mice for six days in group CON or group DEX,respectively.Mice were euthanized after 30 days and the tumor size was recorded.Results Dexmedetomidine alone increased the cell viability of Huh-7 cells,and further improved the cell viability induced by OGD/R injury.On the other hand,the ability of migration was significantly increased by dexmedetomidine.Dexmedetomidine increased the expression of SIRT1 in normal condition and in OGD/R injury.However,there was no difference in tumor burden between group DEX and group CON.Conclusion Dexmedetomidine can affect the proliferation and migration of Huh-7 human hepatocellular carcinoma cells,in which SIRT1 may participate.

关 键 词：右美托咪定 肝癌 HUH-7细胞 SIRT1 

分 类 号：R735.7[医药卫生—肿瘤] R73-37[医药卫生—临床医学]