干扰AKAP12调控G1/S期促进乳腺癌MCF-7细胞增殖  

Interfering with AKAP12 promotes proliferation of breast cancer MCF-7 cells by regulating G1/S phase

作　　者：梁瑞鹏 程倩 陆春花[2] 李可[3] 于恒恒 徐彬[2] 袁顺杰 刘维薇[4] 林清 Liang Ruipeng;Cheng Qian;Lu Chunhua(Medical College^Anhui University of Science and Technology,Huainan 232000;Dept of Radiation Oncology,Tenth People's Hospital,Tongji University,Shanghai 200072)

机构地区：[1]安徽理工大学医学院,淮南232000 [2]同济大学第十人民医院肿瘤放射治疗科,上海200072 [3]同济大学第十人民医院检验科,上海200072 [4]同济大学第十人民医院中心实验室,上海200072

出　　处：《安徽医科大学学报》2020年第7期1041-1045,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81572061)。

摘　　要：目的研究干扰A型激酶锚定蛋白12(AKAP12)对人乳腺癌MCF-7细胞增殖能力的影响,并初步探讨其机制。方法采用携带干扰AKAP12核苷酸序列(shRNA-AKAP12)、空白对照(shRNA-NC)的慢病毒转染乳腺癌MCF-7细胞。Western blot检测转染后细胞AKAP12蛋白表达;CCK-8实验、克隆形成检测增殖能力;Western blot检测细胞周期相关蛋白激酶抑制蛋白(p21、p27)以及细胞周期进展蛋白CyclinD1表达。结果与shRNA-NC细胞相比较,shRNA-AKAP12细胞AKAP12蛋白表达量减少,CCK-8实验以及克隆形成结果显示,shRNA-AKAP12细胞增殖能力明显增强(P<0.000 1、P<0.001),与shRNA-NC细胞相比,shRNA-AKAP12细胞周期相关蛋白激酶抑制蛋白(p21、p27)表达下调,而细胞周期进展蛋白CyclinD1表达上调。结论研究表明干扰AKAP12基因可增加MCF-7细胞增殖能力,干扰AKAP12表达加快MCF-7细胞G1/S期转换,对于雌激素受体阳性乳腺癌临床治疗和预后评估有一定价值。Objective To investigate the effect of interference with AKAP12 on the proliferation ability of human breast cancer MCF-7 cells and to explore its mechanism.Methods MCF-7 cells were exogenously transfected lentivirus with shRNA-AKAP12 and shRNA-NC, respectively. Western blot was conducted to examine the expression of AKAP12 protein in transfected cells. The proliferation ability of transfected cells were measured by CCK-8 assay and cell plate cloning assay. Western blot was used to detect the protein expression levels of cyclin-dependent kinase(CDK) inhibitors(p21, p27) and cell cycle progression-related CyclinD1.Results Compared to shRNA-NC cells, the expression of AKAP12 protein in shRNA-AKAP12 cells decreased. CCK-8 assay and colony formation assay showed that the cell proliferation ability of shRNA-AKAP12 cells increased significantly(P<0.000 1, P<0.001). Compared to the shRNA-NC cells, the expression of the cyclin-dependent kinase(CDK) inhibitors(p21, p27) were down-regulated in the shRNA-AKAP12 cells, while the cell cycle progression protein(CyclinD1) was up-regulated.Conclusion The study indicated that interference with AKAP12 gene could increase the proliferation ability of MCF-7 cells. It accelerated the G1-S phase transition of MCF-7 cells after interference with AKAP12 gene. Thus it is available to clinical treatment and prognosis evaluation of estrogen receptor-positive breast cancer.

关 键 词：A型激酶锚定蛋白12 乳腺癌 细胞周期 

分 类 号：R730[医药卫生—肿瘤]