消乳散结汤单药对人乳腺癌MCF-7/ADR细胞耐药性影响的初步研究  

Experimental study on the effect of single drugs of xiaorusanjietang on human brest cancer drug-resistance cell line MCF-7/ADR

作　　者：李晓梅 宋金艳 陈芳 徐林 李天禹 罗清 Li Xiaomei;Song Jinyan;Chen Fang(Dept of Cancer Research Laboratory,The Affiliated Hospital of Zunyi Medical University,Zunyi 563000;Dept of Traditional Chinese Medicine,The Affiliated Hospital of Zunyi Medical University,Zunyi 563000)

机构地区：[1]遵义医科大学附属医院肿瘤研究室,遵义563000 [2]遵义医科大学附属医院中医科,遵义563000

出　　处：《安徽医科大学学报》2020年第7期1084-1088,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81860469);贵州省卫生计生委科学技术基金(编号:gzwjkj2017-1-024);遵义市科技创新人才及团队培养项目(编号:遵科合人(2014)9号);贵州省科技合作计划项目(编号:黔科合LH字[2015]7485号);遵义市科技计划项目(编号:遵市科合社字(2014)98号)。

摘　　要：目的比较消乳散结汤的五种单药-熟地黄、白芍、栀子、半枝莲以及浙贝母对人乳腺癌耐阿霉素细胞MCF-7/ADR耐药敏感性的影响。方法炮制熟地黄、白芍、栀子、半枝莲以及浙贝母五种单药水剂,并进行了冷冻抽干浓缩,各单药冻干粉用磷酸盐缓冲液(PBS)溶解为1 mg/ml储液;MTT法检测各单药对MCF-7/ADR细胞的无毒杀伤剂量;以无毒剂量作为耐药逆转浓度预处理MCF-7/ADR细胞后,加入阿霉素,用MTT法检测各单药对细胞的耐药性是否具有逆转作用。结果五种单药分别以无毒性的低剂量(1μg/ml)和高剂量(10μg/ml)浓度处理MCF-7/ADR细胞,未观察到单药熟地黄、白芍、栀子和半枝莲对细胞耐药性的逆转作用。而单药浙贝母处理时,阿霉素具有杀伤效应(P<0.05),并且,与不同浓度阿霉素联合,观察到剂量依赖的逆转效应。结论消乳散结汤中单药浙贝母对人乳腺癌阿霉素耐药细胞MCF-7/ADR的耐药性具有逆转作用,且逆转效应具有剂量相关性。Objective To compare the effects of five single drugs of xiaorusanjietang-radix rehmanniae praeparata, paeonia lactiflora, fructus gardeniae, sculellaria barbata and fritillaria thunbergii on the sensitivity of drug resistance to adriamycin resistant human breast cancer cell line MCF-7/ADR. Methods Radix rehmanniae praeparata, paeonia lactiflora, fructus gardeniae, sculellaria barbata and fritillaria thunbergii were prepared and distilled. The freeze-dried powder of each medicine was dissolved into 1 mg/ml liquid storage solution with phosphate buffer saline(PBS). The methyl thiazolyl tetrazolium(MTT) assay was used to determine the non-toxic and lethal dose of each drug to MCF-7/ADR cells. After pretreatment of MCF-7/ADR cells with non-toxic dose as the reverse concentration of drug resistance, adriamycin was added and MTT method was used to detect whether the single drug had a reverse effect on cell resistance. Results MCF-7/ADR was treated with non-toxic low dose(1 μg/ml) and high dose(10 μg/ml), respectively, and no single drug radix rehmanniae praeparata, paeonia lactiflora, fructus gardeniae and sculellaria barbata were observed to reverse cell resistance. However, when treated with fritillaria thunbergii alone, adriamycin had a killing effect(P<0.05), and the dose-dependent reversal effect was observed when combined with adriamycin of different concentrations. Conclusion The single drug fritillaria thunbergii in xiaorusanjietang has a reversal effect on the drug resistance of MCF-7/ADR cells, and the reversal ability is dose-dependent.

关 键 词：消乳散结汤单药 乳腺癌 多药耐药 耐药逆转 

分 类 号：R73-3[医药卫生—肿瘤]