白果中植物甾醇的提取工艺优化及体外释放研究  

Extraction Optimization and in Vitro Release of Phytosterol from Ginkgo

作　　者：刘真阳 张碧霄 李非凡 梅晓宏[1] LIU Zhen-yang;ZHANG Bi-xiao;LI Fei-fan;MEI Xiao-hong(College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国食物与营养》2020年第8期19-23,共5页Food and Nutrition in China

摘　　要：目的:优化白果中植物甾醇的提取并考察其体外释放情况。方法:以白果为原料,以乙醇为提取溶剂,使用超声波辅助提取法,在单因素试验的基础上,通过正交试验优化了白果中植物甾醇的提取工艺,进一步通过体外模拟消化试验,分析了添加油脂、热处理方式、物料粉碎程度对甾醇体外释放的影响。结果:超声时间、料液比、超声功率均影响甾醇的提取率。超声功率对提取率具有显著影响,最佳工艺为超声时间6 min、料液比1∶15 g/mL、超声功率350W,此时可得最大甾醇提取率为0.878 mg/(g·dw)。体外模拟消化试验证明,添加油脂、热处理方式、物料粉碎程度均对植物甾醇的体外释放具有显著影响,当使用微波加热处理时,甾醇的释放情况最佳。Objective To optimize the extraction of phytosterol from ginkgo and further evaluate in vitro release of phytosterol.Method Ultrasonic-assisted extraction was used and orthogonal test was applied for optimization.Simulated digestion experiment was carried out to analyze the effect of additional oil,heat treatment and grinding on in vitro release of phytosterol.Result Based on the single factor experiment,extraction time,solid-liquid ratio,and ultrasonic power could affect extraction efficiency,while only ultrasonic power showed significant influence according to orthogonal test.Besides,the optimization was extraction time 6 min,the ratio of material to solvent 1∶15 g/mL,and ultrasonic power 350 W.Under these conditions,the theoretic yield was 0.878 mg/(g·dw).Simulated digestion experiment evidenced that additional oil,heat treatment and thorough grinding facilitated phytosterol release,and microwave heat-treatment showed the best release ratio.

关 键 词：白果 植物甾醇 模拟消化 

分 类 号：TQ28[化学工程—有机化工]