健脾法联合肠内营养对溃疡性结肠炎大鼠肠道菌群的影响  

Effect of combination of invigorating spleen recipes and enteral nutrition on intestinal flora in rats with ulcerative colitis

作　　者：潘丹峰[1] 苍玉珍[1] 沈峰[1] 林寒潇 Pan Danfeng;Cang Yuzhen;Shen Feng;Lin Hanxiao(Department of Clinical Nutrition,Guangzhou First People’s Hospital,Guangzhou 510180,China)

机构地区：[1]广州市第一人民医院临床营养科,广州510180

出　　处：《新医学》2020年第9期691-696,共6页Journal of New Medicine

基　　金：广东省中医药局科研项目(20181195)。

摘　　要：目的探索健脾法联合肠内营养(EN)对溃疡性结肠炎(UC)大鼠肠道菌群的影响。方法选用60只SD大鼠,使用葡聚糖硫酸钠诱导建立UC大鼠模型。UC模型建立之后,将SD大鼠分为空白组、模型组、EN组、健脾组和EN+健脾组,每组12只,分组干预。记录大鼠体质量变化情况和疾病活动指数(DAI),观察结肠长度和组织学变化,最后用16S rDNA测序法测定大鼠粪便中肠道细菌的多样性和相对丰度。结果与模型组相比,EN组和EN+健脾组UC症状显著改善,UC大鼠体质量降低程度和DAI增加程度显著减小,结肠缩短程度和组织学损伤评分得到改善,菌群多样性升高,乳酸杆菌科和双歧杆菌科相对丰度提高,肠杆菌和拟杆菌科相对丰度下降(P均<0.05或0.05/10)。与单纯的EN组相比,EN和健脾方的联合干预可进一步改善UC症状,提高菌群多样性,增加乳酸杆菌科和双歧杆菌科的相对丰度,降低肠杆菌和拟杆菌科的相对丰度(P均<0.05/10)。结论健脾法联合EN能有效地改善UC大鼠的肠道菌群。Objective To evaluate the effect of combination of invigorating spleen recipe and enteral nutrition(EN)on the intestinal flora in SD rat models with ulcerative colitis(UC).Methods Sixty SD rat models with UC were established by dextran sodium sulfate.After the UC model was established,all SD rats were randomly divided into the blank,model,EN,invigorating spleen and EN+invigorating spleen groups,12 rats for each group.The changes in body weight and disease activity index(DAI)of the rats were recorded,and the length and histological changes of the colon were observed.Finally,the diversity and relative abundance of microbial bacteria in the intestine of rats were evaluated by the 16S rDNA sequencing method.Results Compared with the model group,the UC-related symptoms were significantly improved in the EN and EN+invigorating spleen groups.The degree of weight loss and DAI increase in the UC rats were significantly reduced,the length of colon and histological scores were remarkably improved,and the bacterial diversity was increased.The relative abundance of Lactobacillus and Bifidobacteriaceae was elevated,whereas that of Enterobacteriaceae and Bacteroideceae was decreased(all P<0.05 or 0.05/10).Compared with the EN alone group,the combined treatment of EN and invigorating spleen further mitigated the UCrelated symptoms,increased the diversity of the flora,enhanced the relative abundance of Lactobacillus and Bifidobacteriaceae,and reduced the relative abundance of Enterobacteriaceae and Bacteroideae(all P<0.05/10).Conclusions The combined treatment of EN and invigorating spleen recipes can effectively improve the intestinal flora in UC rats.

关 键 词：健脾法 肠内营养 溃疡性结肠炎 肠道菌群 

分 类 号：R574.62[医药卫生—消化系统]