miR-129-5p、p62和STAT3在结肠癌组织中的表达及临床意义  

Expressions and clinical significances of miR-129-5p,p62 and STAT3 in colon cancer

作　　者：潘晟[1] 黄林飞[1] 夏甘霖 陶艳娥 徐竞[1] 李俊[1] 梅文超 PAN Sheng;HUANG Lin-fei;XIA Gan-lin;TAO Yan-e;XU Jing;LI Jun;MEI Wen-chao(Department of Gastrointestinal Surgery,Puren Hospital Affiliated to Wuhan University of Science and Technology,Wuhan 430000,China)

机构地区：[1]湖北省武汉市武汉科技大学附属普仁医院胃肠外科,湖北武汉430000

出　　处：《中国现代普通外科进展》2020年第8期600-605,共6页Chinese Journal of Current Advances in General Surgery

基　　金：湖北省卫生健康科研基金资助(基金编号:WJ2019H225)。

摘　　要：目的:探讨结肠癌组织中miR-129-5p、p62和信号转导与转录激活因子3(STAT3)表达水平与结肠癌进展及预后的关系。方法:选取2013年6月-2016年6月本院收治的113例结肠癌患者为研究对象,术中收集结肠癌组织及癌旁组织(距癌组织≥5 cm)。实时荧光定量(qRT-PCR)法检测结肠癌组织中miR-129-5p、p62 mRNA、STAT3 mRNA表达水平,免疫组织化学染色法检测p62及STAT3蛋白表达水平。分析三者表达与结肠癌患者临床病理特征及预后关系。结果:结肠癌组织中miR-129-5p表达水平显著低于癌旁组织(P<0.05),p62和STAT3的mRNA表达水平及蛋白高表达率均显著高于癌旁组织(P均<0.05)。miR-129-5p和p62蛋白表达水平与分化程度、浸润深度、TNM分期及淋巴结转移有关(P均<0.05),STAT3蛋白表达水平与浸润深度、TNM分期及淋巴结转移有关(P均<0.05)。miR-129-5p高表达组3年累积生存率显著高于miR-129-5p低表达组(P<0.05)。p62及STAT3蛋白高表达组患者3年累积生存率显著低于p62及STAT3蛋白低表达组(P<0.05)。结肠癌组织中miR-129-5p与STAT3 mRNA表达水平呈负相关(r=-0.390,P<0.05),p62 mRNA与STAT3 mRNA表达水平呈正相关(r=0.402,P<0.05)。结论:在结肠癌组织中miR-129-5p表达下调,p62、STAT3 mRNA水平及蛋白高表达率均上调,三者表达水平均与浸润深度、TNM分期、淋巴结转移及3年累积生存率有关,可能成为结肠癌预后标志物,为结肠癌临床治疗提供指导。Objective:To detect the expression levels of miR-129-5p,p62 and STAT3 in colon cancer tissues,and to analyze their relationships with the progression and prognosis of colon cancer.Methods:113 patients with colon cancer admitted to our hospital from June 2013 to June 2016 were selected as the study subjects,the colon cancer tissues and adjacent tissues(≥5 cm from cancer tissue)were collected during the operation.Real-time fluorescence quantitative analysis(qRT-PCR)was used to detect the expression levels of miR-129-5p,p62 mRNA and STAT3 mRNA,immunohistochemical staining was used to detect the expression levels of p62 and STAT3 protein.Their relationships with clinicopathological characteristics and prognosis of patients with colon cancer were analyzed.Results:The expression level of miR-129-5p in colon cancer tissues was significantly lower than that in adjacent tissues(P<0.05),and the expression levels of p62 and STAT3 mRNA and the high expression rate of protein were significantly higher than those in adjacent tissues(P<0.05).The expression levels of miR-129-5p and p62 protein were correlated with the degree of differentiation,depth of invasion,TNM stage and lymph node metastasis(all P<0.05),and the expression level of STAT3 was correlated with depth of invasion,TNM stage and lymph node metastasis(all P<0.05).The 3-year cumulative survival rate of the miR-129-5p high-expression group was significantly higher than that of the miR-129-5p low-expression group(P<0.05).The 3-year cumulative survival rate of expression of p62 and STAT3 protein high-expression group was significantly lower than that of p62 and STAT3 protein low-expression group(P<0.05).There was a negative correlation between the expression of miR-129-5p and the expression of STAT3 mRNA in colon cancer(r=-0.390,P<0.05),and there was a positive correlation between the expressions of p62 mRNA and STAT3 mRNA(r=0.402,P<0.05).Conclusion:Compared with the adjacent tissues,the expression of miR-129-5p is down-regulated in colon cancer tissues,the levels of

关 键 词：结肠肿瘤 miR-129-5p P62 信号转导与转录激活因子3 

分 类 号：R735.35[医药卫生—肿瘤]