高效异养硝化-好氧反硝化菌株TS-1筛选及降解特性  

Characterizing the heterotrophically nitrifying and aerobically denitrifying TS-1 strain

作　　者：李海红[1] 佟欣宇 宦臣臣 闫志英[2] LI Haihong;TONG Xinyu;HUAN Chenchen;YAN Zhiying(College of Environmental and Chemical Engineering,Xi’an Polytechnic University,Xi’an 710000,China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China)

机构地区：[1]西安工程大学环境与化学工程学院,西安710000 [2]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2020年第4期791-799,共9页Chinese Journal of Applied and Environmental Biology

基　　金：四川省重大科技专项课题(2018SZDZX0024);中国科学院战略生物资源计划(KFJ-BRP-009);四川省重点研发项目(2019YFS0052)资助。

摘　　要：从实验室定向驯化的活性污泥中分离筛选出一株具有异养硝化-好氧反硝化功能的菌株TS-1.通过生理生化及16S r RNA基因序列鉴定其为脱氮副球菌,通过单因素和正交实验对其去除NH4+-N的最佳条件进行优化,并通过对比进一步探究其在不同氮源条件下对各形态无机氮的去除规律.结果表明该菌株最适碳源为丁二酸钠,最佳C/N为15,最佳接种量为5%,最适温度为30℃、p H为8.0.以初始浓度约为100 mg/L的NH4+-N、NO3--N和NO2--N分别为单一氮源时,菌株TS-1对各形态氮的去除率为97.49%、100%和95.94%;维持各形态氮初始浓度不变,将其两两混合时发现混合氮源中若包含NO2--N会使菌株OD600值达到最大值所用时间延长,氮源中含有NH4+-N会降低菌株对其他形态氮源的去除率,以及NO3--N的添加会使菌株对NH4+-N的去除能力降低;3种形态氮源同时存在的条件下,该菌对各氮源去除能力由强至弱为NO2--N>NH4+-N>NO3--N.本研究从活性污泥中分离筛选出一株具有高效异养硝化-好氧反硝化功能的菌株TS-1,通过研究碳源、氮源、温度、p H得到了最佳降解条件,可为废水短程脱氮提供参考.(图9表4参37)In this study,the TS-1 strain with its heterotrophic nitrification and aerobic denitrification functions was isolated from an activated sludge that was acclimated in a laboratory.According to its physiological and biochemical characteristics as well as the 16S rRNA gene sequence,it was identified as Paracoccus denitrificans.Through single factor and orthogonal experiments,the NH4+-N removal conditions were optimized,while comparing the different forms and sources of inorganic nitrogen.The strain’s optimal carbon source,C/N ratio,inoculation amount,temperature,and p H were identified as sodium succinate,15,5%,30℃,and 8.0,respectively.In addition,the individual removal rates of NH4+-N,NO3--N,and NO2--N by the TS-1 strain using an initial concentration of approximately 100 mg/L,were 97.49%,100%,and 95.94%,respectively.When the initial concentration of the nitrogen forms was unchanged,but NO2--N was included,the time required for the strain to reach maximum optical density at 600 nm was prolonged,while including NH4+-N reduced the removal rate of other nitrogen sources using pressure,but adding NO3--N reduced this ability.Under the condition that the three forms of nitrogen can exist simultaneously,the bacteria’s removal ability of each nitrogen source was NO2--N>NH4+-N>NO3--N.Hence,the highly efficient heterotrophically nitrifying and aerobically denitrifying TS-1 strain was successfully isolated from an activated sludge,in which the optimal degradation conditions were identified by examining the carbon source,nitrogen source,temperature,and p H,to provide a reference for the shortrange denitrification of wastewater.

关 键 词：异养硝化 好氧反硝化 Paracoccus denitrificans 混合氮源 生物脱氮 

分 类 号：X172[环境科学与工程—环境科学]