血清可溶性间皮素相关蛋白早期诊断大鼠恶性胸膜间皮瘤的实验研究  

Experimental Study on Diagnosis of Rats Malignant Pleural Mesothelioma with Serum Soluble Mesothelin-related Peptides

作　　者：江杰[1] 文亮[1] 吴莉[1] 谢晓洁[1] 赵雯[1] 韩丹[1] Jiang Jie;Wen Liang;Wu Li(Medical Imaging Department,The First Affiliated Hospital,Kunming Medical University,Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院医学影像科,650032

出　　处：《医学研究杂志》2020年第9期32-35,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81460264,81960310);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2014FA013,2018FE001-041);云南省高层次卫生健康技术人才培养专项基金资助项目(H-2018007)。

摘　　要：目的通过定量检测大鼠恶性胸膜间皮瘤血清中SMRP的表达情况,探讨早期诊断恶性胸膜间皮瘤(MPM)的价值,为高危人群筛查、早期诊断提供理论依据及实验基础。方法采用ELISA法定量测定大鼠MPM SMRP的表达情况,分析MPM不同分期、不同病理类型、不同分化程度的SMRP表达差异,应用受试者工作特征曲线(ROC曲线)获取早期诊断MPM的诊断效能。结果不同分期、不同分化程度的MPM SMRP表达比较差异有统计学意义(P<0.05),分期越高,SMRP表达越高;分化程度越低,SMRP表达越高。不同病理类型表达比较差异无统计学意义(P>0.05)。对T 1、T 2、T 3和T 4分期诊断AUC分别为0.83、0.86、0.92和0.85,敏感度、特异性分别为100.0%、88.2%,100.0%、85.7%,71.4%、100.0%和87.5%、100.0%,诊断阈值分别为1.90、4.53、8.73和11.97mmol/ml。对N 0、N 1、N 2分期诊断,AUC值为0.59、0.84、0.92,敏感度、特异性分别为100.0%、100.0%,47.1%、100.0%和64.0%、100.0%。对M 1分期诊断,AUC=0.88,敏感度、特异性分别为75.0%、100.0%。结论血清SMRP表达水平对筛查、早期诊断MPM具有重要价值,可作为MPM高危人群筛查的一项重要生化指标。Objective To investigate the value on early diagnosis of experimental rats malignant pleural mesothelioma according to the serum level of soluble mesothelin-related peptides(SMRP),providing theoretical basis and experimental basis for early screening high-risk groups.Methods The expression of MPM SMRP in rats was determined by enzyme-linked immunosorbent assay(ELISA)and the differences in the SMRP expression were analyzed in different tumour stages,pathological types and the degree of histological differentiation.The ROC curve was used to obtain the diagnostic efficiency of early diagnosis of MPM.Results There were statistically significant differences in the SMRP expression in MPM different stages and degrees of differentiation(P<0.05).The higher the stage,the higher the expression of SMRP.The lower the degree of differentiation,the higher the SMRP expression.However,there was no significant difference in expression among different pathological types(P>0.05).The AUC value of SMRP for T 1,T 2,T 3,T 4 were 0.83,0.86,0.92,0.85,the sensitivity and specificity were 100.0%,88.2%,100.0%,85.7%,71.4%,100.0%,87.5%,100.0%,respectively,and the diagnostic threshold was 1.90,4.53,8.73,11.97mmol/ml.Moreover,the AUC value of SMRP for N 1,N 2,N 3 were 0.59,0.84,0.92,respectively,and the sensitivity and specificity were 100.0%,100.0%,47.1%,100.0%,64.0%,100.0%,respectively.The AUC value of SMRP for M 1 were 0.88,and the sensitivity and specificity were 75.0%,100.0%,respectively.Conclusion SMRP can be used as an important biochemical indicator for screening of high-risk population of MPM.

关 键 词：间皮素 间皮瘤 可溶性间皮素相关蛋白 恶性胸膜间皮瘤 

分 类 号：R734.3[医药卫生—肿瘤]