桑树SPL转录因子家族全基因组鉴定及特征分析  

Genome-Wide Identification and Characterization of Squamosa Promoter Binding Protein-like Transcription Factors in Morus notabilis

作　　者：韩利红 刘潮 赵明玉 胡丽娟 胡玉霜 HAN Lihong;LIU Chao;ZHAO Mingyu;HU Lijuan;HU Yushuang(College of Biological Resource and Food Engineering,Qujing Normal University/Center for Yunnan Plateau Biological Resources Protection and Utilization/Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yungui Plateau,Qujing 655011,China)

机构地区：[1]曲靖师范学院生物资源与食品工程学院/云南高原生物资源保护与利用研究中心/云南省高校云贵高原动植物多样性及生态适应性进化重点实验室,云南曲靖655011

出　　处：《河南农业科学》2020年第9期33-42,共10页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31860005);云南省地方本科高校基础研究联合专项(2017FH001-034);国家级大学生创新创业训练计划项目(201710684012,201810684010)。

摘　　要：SQUAMOSA启动子结合类蛋白质(SQUAMOSA promoter binding protein-like,SPL)是一类植物生长发育和应对胁迫相关的重要转录因子。利用生物信息学方法,从川桑(Morus notabilis)基因组中鉴定到15个SPL基因,进化分析将其归为8个聚类组,其具有保守的基因结构和氨基酸基序,在拟南芥和水稻中存在多个直系同源基因,预测到7个MnSPLs基因的表达受miR156/157调控,MnSPLs在桑树多个组织中检测到表达,其中6个基因在5类组织中均有较高表达量,部分基因主要在冬芽和雄花中有较高表达量,MnSPL基因存在组织表达特异性,在桑树生长发育过程中发挥重要作用。SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)protein is plant-specific transcription factor,play vital roles in the regulatory responses of plant growth,development and stress.In this study,15 SPL genes were identified from Morus notabilis.MnSPLs transcription factors were clustered into eight groups.Gene structure and motif analysis indicated that most MnSPLs had relatively conserved exon-intron arrangements and amino acids motif composition.Several paralogs and orthologs were predicted within the MnSPLs and along with Arabidopsis thaliana and Oryza sativa SPLs.Seven MnSPLs were predicted to be targets of miR156/157.MnSPLs were expressed differentially in various tissues of M.notabilis,six genes of them were highly expressed in five tissue,some genes were mainly highly expressed in winter buds and male flowers.MnSPL genes tissue-specific expression,and played important role in the growth and development of mulberry.

关 键 词：桑树 川桑 SPL基因 转录因子 miR156/157 基因表达 

分 类 号：Q78[生物学—分子生物学]