环状RNA CDR1as与miR-7在癫痫患者血浆中的表达及与脑电图异常的关系分析  

The expressions of circled RNA CDR1as and miR-7 in epileptic patients and their relationships with abnormal EEG

作　　者：韩永凯[1] 李斯娜[2] 张萍[1] 李静[3] 王旭生[1] 张帆[1] 杜爱玲[1] 张留莎[1] 宋景贵[1] HAN Yong-kai;LI Si-na;ZHANG Ping;LI Jing;WANG Xu-sheng;ZHANG Fan;DU Ai-ling;ZHANG Liu-sha;SONG Jing-gui(Department of Neurology,the Second Affiliated Hospital of Xinxiang Medical College,Xinxiang 453002,China;Department of Emergency,the Second Affiliated Hospital of Xinxiang Medical College,Xinxiang 453002,China;Department of Neurology,the Third Affiliated Hospital of Xinxiang Medical College)

机构地区：[1]河南省新乡医学院第二附属医院神经内科,453002 [2]河南省新乡医学院第二附属医院急诊科,453002 [3]河南省新乡医学院第三附属医院神经内科

出　　处：《天津医药》2020年第9期871-875,共5页Tianjin Medical Journal

基　　金：2018年度河南省医学科技攻关计划项目(2018020374)。

摘　　要：目的探讨环状RNA小脑变性相关蛋白1反义转录物(CDR1as)与微小RNA-7(miR-7)在癫痫患者血浆中的表达及与脑电图异常的关系。方法选取2016年12月—2019年12月在新乡医学院第二附属医院门诊及住院的癫痫患者87例为观察组,并根据脑电图结果分为正常组(6例)、轻度异常组(18例)、中度异常组(37例)及重度异常组(26例);选取同期健康体检者90例为对照组。实时荧光定量PCR(qPCR)法检测血浆CDR1as、miR-7水平,酶联免疫吸附测定(ELISA)法检测血浆白细胞介素(IL)-2、肿瘤坏死因子(TNF)-α和IL-1β水平;Pearson法分析癫痫患者血浆CDR1as、miR-7水平与IL-2、TNF-α和IL-1β水平的相关性。结果对照组、正常组、轻度异常组、中度异常组、重度异常组血浆CDR1as、IL-2、TNF-α和IL-1β水平总体呈升高变化,miR-7水平总体呈降低变化,差异均有统计学意义(P<0.05)。癫痫患者血浆CDR1as水平与IL-2、TNF-α和IL-1β水平呈正相关(P<0.05),miR-7水平与IL-2、TNF-α和IL-1β水平呈负相关(P<0.05)。结论CDR1as、miR-7在癫痫患者血浆中分别呈高表达、低表达,与炎症因子水平、脑电图异常程度密切相关,可能通过影响炎症反应引起脑部异常放电。Objective To investigate the expressions of circular RNA cerebellar degeneration-related protein 1 antisense(CDR1as)and microRNA-7(miR-7)in the plasma of epileptic patients and their relationships with abnormal EEG.Methods A total of 87 epileptic patients treated and hospitalized in the Second Affiliated Hospital of Xinxiang Medical College from December 2016 to December 2019 were selected as the study objects(observation group).According to the electroencephalograph(EEG)results,patients were divided into normal group(n=6),mild abnormal group(n=18),moderate abnormal group(n=37)and severe abnormal group(n=26).In addition,90 healthy people in the same period were selected as the control group.The plasma levels of CDR1as and miR-7 were detected by real-time fluorescent quantitative PCR(qPCR).The plasma levels of interleukin 2(IL-2),tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were detected by enzyme-linked immunosorbent assay(ELISA).Pearson´s method was used to analyze the correlation between the levels of CDR1as,miR-7 and the levels of IL-2,TNF-α and IL-1β.Results The levels of CDR1as,IL-2,TNF-α and IL-1β were significantly increased in the normal group,mild abnormal group,moderate abnormal group and severe abnormal group(P<0.05),while the level of miR-7 was significantly decreased(P<0.05).There was a positive correlation between the plasma levels of CDR1as and IL-2,TNF-α and IL-1β in epileptic patients(P<0.05).There was a negative correlation between the level of miR-7 and IL-2,TNF-α and IL-1β(P<0.05).Conclusion The plasma expressions of CDR1as and miR-7 are higher and lower in epileptic patients,respectively,and they are closely related to the levels of inflammatory factors and the abnormal degree of EEG,which may cause abnormal discharge of brain by influencing the inflammatory response.

关 键 词：癫痫 微RNAs 脑电描记术 白细胞介素2 肿瘤坏死因子α 白细胞介素1Β 小脑变性相关蛋白1反义转录物 miR-7 

分 类 号：R742.1[医药卫生—神经病学与精神病学]