马铃薯闽薯1号茎尖脱毒与快速繁殖技术研究  

Study on Stem Tip Virus-free and Rapid Propagation of Potato Minshu 1

作　　者：黄萍萍[1] 曾军[1] 梁金平[1] 张川[1] 张志勇[1] 周美玲[1] HUANG Ping-ping;ZENG Jun;LIANG Jin-ping;ZHANG Chuan;ZHANG Zhi-yong;ZHOU Mei-ling(Longyan Institute of Agricultural Sciences,Longyan Fujian 364000)

机构地区：[1]龙岩市农业科学研究所,福建龙岩364000

出　　处：《现代农业科技》2020年第18期46-47,50,共3页Modern Agricultural Science and Technology

基　　金：福建省科技计划项目农业引导性(重点)项目(2018N0062);福建省科技厅农业引导性(重点)项目(2017N0047)。

摘　　要：为了提高马铃薯闽薯1号的种薯质量,以满足市场的需求,开展马铃薯闽薯1号脱毒试验。结果表明,外植体表面灭菌氯化汞体积浓度为0.1%、消毒时间为8 min的消毒效果好,外植体成活率达90.5%;剥取闽薯1号茎尖,在MS+6-BA 1.00 mg/L+NAA 0.1 mg/L培养基中培养30 d,形成闽薯1号试管苗,试管苗通过变温热处理培养21 d,使部分病毒钝化死亡,之后将试管苗剥取大小1 mm茎尖进行培养,经检测不带病毒,对脱毒苗进行快速繁殖。脱毒苗快繁的较佳培养基配方为MS+6-BA 0.10 mg/L+NAA 0.10 mg/L。In order to improve the seed tuber quality of potato Minshu 1 and meet the market demand,the experiment of potato Minshu 1 virus-free was conducted.The results showed that the disinfection effect was good when the concentration of mercury chloride was 0.1%and the disinfection time was 8 min,and the survival rate of the explants was 90.5%.The stem tip of Minshu 1 was peeled off and cultured in MS+6-BA 1.00 mg/L+NAA 0.1 mg/L for 30 days to form the tube seedling.The tube seedling was incubated for 21 days with heat treatment whose temperature was changed,and some viruses were inactivated and died.After that,the stem tip of the tube seedling was cultured with the size of 1 mm.After detection,the tube seedling was rapidly propagated without viruses.The optimal medium formula for rapid propagation of virus-free seedlings was MS+6-BA 0.10 mg/L+NAA 0.10 mg/L.

关 键 词：马铃薯 闽薯1号 茎尖脱毒技术 快速繁殖 

分 类 号：S532[农业科学—作物学]