膀胱尿路上皮癌组织中SRSF1及Caspase-3的表达水平及临床意义  

Expression Level and Clinical Significance of SRSF1 and Caspase-3 in Bladder Urothelial Carcinoma

作　　者：宰国真[1] 王旭东[1] 马梅[1] 李彦伟[1] ZAI Guo-zhen;WANG Xu-dong;MA Mei;LI Yan-wei(Department of Pathology,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第五附属医院病理科,河南郑州450052

出　　处：《河南医学研究》2020年第25期4635-4638,共4页Henan Medical Research

摘　　要：目的探讨膀胱浸润性尿路上皮癌组织中丝氨酸/精氨酸富有剪接因子1(SRSF1)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)的表达水平及临床意义。方法选取2017年3月至2019年6月郑州大学第五附属医院收集的92例膀胱浸润性尿路上皮癌手术或活检标本为观察组,选取同期20例病理检查结果为正常膀胱尿路上皮组织的活检标本为对照组。采用免疫组化法检测对照组、观察组中SRSF1及Caspase-3的表达情况,分析观察组Caspase-3与SRSF1的相关性。结果观察组SRSF1阳性表达率高于对照组(P<0.05)。复发性浸润性尿路上皮癌组织SRSF1阳性表达率高于原发性癌(P<0.001)。观察组Caspase-3阳性表达率低于对照组(P<0.05)。观察组Caspase-3与SRSF1的表达呈负相关(P<0.05)。结论SRSF1与膀胱浸润性尿路上皮癌的发生、复发关系密切,膀胱浸润性尿路上皮癌组织中Caspase-3与SRSF1表达呈负相关,SRSF1高表达与Caspase-3低表达对膀胱浸润性尿路上皮癌患者病情进展起促进作用。Objective To investigate the expression level and clinical significance of serine/arginine rich splicing factor 1(SRSF1)and aspartate specific caspase-3(Caspase-3)in invasive urothelial carcinoma of the bladder.Methods Ninety-two surgical or biopsy specimens of invasive urothelial carcinoma of the bladder collected in the Fifth Affiliated Hospital of Zhengzhou University from March 2017 to June 2019 were selected as observation group,and twenty biopsy specimens with normal bladder urothelial tissue in the same period were selected as control group.The immunohistochemical method was used to analyze the expressions of SRSF1 and Caspase-3 in control group and observation group.The correlation between Caspase-3 and SRSF1 in observation group was analyzed.Results The positive expression rate of SRSF1 in observation group was higher than that in control group(P<0.05).The positive expression rate of SRSF1 in recurrent infiltrating urothelial carcinoma tissue was higher than that in primary carcinoma(P<0.001).The positive expression rate of Caspase-3 in observation group was lower than that in control group(P<0.05).The expression of Caspase-3 and SRSF1 in observation group was negatively correlated(P<0.05).Conclusion SRSF1 is closely related to the occurrence and recurrence of invasive urothelial carcinoma of the bladder.There is a negative correlation between the expression of Caspase-3 and SRSF1 in invasive urothelial carcinoma of the bladder.The high expression of SRSF1 and low expression of Caspase-3 may promote the progression of invasive urothelial carcinoma of the bladder.

关 键 词：膀胱浸润性尿路上皮癌 丝氨酸/精氨酸富有剪接因子1 天冬氨酸特异性半胱氨酸蛋白酶-3 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]