Highly efficient synthesis of(R)-1,3-butanediol via anti-Prelog reduction of 4-hydroxy-2-butanone with absolute stereoselectivity by a newly isolated Pichia kudriavzevii  

作　　者：Han Zu Hui Zhang Anwen Fan Jie Gu Yao Nie Pengjie Luo Yan Xu 

机构地区：[1]School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education,Jiangnan University,Wuxi 214122,China [2]State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China [3]China National Center for Food Safety Risk Assessment,NHC Key Laboratory of Food Safety Risk Assessment,Beijing 100022,China

出　　处：《Chinese Journal of Chemical Engineering》2020年第8期2160-2166,共7页中国化学工程学报（英文版）

基　　金：Financial supports from the National Key R&D Program of China(2018YFC1604100);the National Natural Science Foundation of China(NSFC)[21676120,31872891];the 111 Project[111-2-06];the Highend Foreign Experts Recruitment Program[G20190010083];the Program for Advanced Talents within Six Industries of Jiangsu Province[2015-NY007];the National Program for Support of Top-notch Young Professionals;the Fundamental Research Funds for the Central Universities[JUSRP51504];the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions;Top-notch Academic Programs Project of Jiangsu Higher Education Institutions;the Jiangsu Province“Collaborative Innovation Center for Advanced Industrial Fermentation”Industry Development Program;the National First-Class Discipline Program of Light Industry Technology and Engineering[LITE2018-09]。

摘　　要：(R)-1,3-butanediol is an important pharmaceutical intermediate, and the synthesis of(R)-1,3-butanediol using green biological methods has recently been of interest for industrial application. Here, a novel strain QC-1 that efficiently transforms 4-hydroxy-2-butanone to(R)-1,3-butanediol was isolated from soil samples. Based on morphological, physiological, and biochemical tests and 5.8 S-internal transcribed spacer sequencing, the strain was identified as Pichia kudriavzevii QC-1. The reaction conditions were optimized to 35 ℃, pH 8.0, rotation speed 200 rpm, and 6:5 mass ratio of glucose to 4-hydroxy-2-butanone. Evaluation of the effects of 4-hydroxy-2-butanone concentrations on yield and cell survival rate showed that 85.60 g·L^-1 product accumulated, with an enantiomeric excess of more than 99%, when 30 g·L^-14-hydroxy-2-butanone was added at 0, 10, and 30 h in a 3-L bioreactor. Thus, strain QC-1 showed excellent catalytic activity and stereoselectivity for the synthesis of(R)-1,3-butanediol from 4-hydroxy-2-butanone.

关 键 词：Asymmetric reduction STEREOSELECTIVITY (R)-1 3-butanediol 4-Hydroxy-2-butanone Whole-cell catalysis 

分 类 号：TQ923[轻工技术与工程—发酵工程]