Inflammation-mediated age-dependent effects of casein kinase 2-interacting protein-1 on osteogenesis in mesenchymal stem cells  

作　　者：Xiao-Guang Tian Fei-Fei Gong Xi Li Fan-Hao Meng Zheng Zhou Hai-Zhong Zhang 

机构地区：[1]Department of Stomatology,Chinese PLA General Hospital,Beijing 100853,China [2]Department of Stomatology,The 960th Hospital of PLA,Taian,Shandong 271000,China [3]Department of AIDS Research,State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China

出　　处：《Chinese Medical Journal》2020年第16期1935-1942,共8页中华医学杂志（英文版）

基　　金：Supported by the grants from the National Key Research and Development Project(No.2017YFB1304300);Conversion Fund of PLA General Hospital(No.2017tm-018);the Clinical Research Support Fund of PLA General Hospital(No.2017fc-tsys-2013).

摘　　要：Background:The casein kinase 2-interacting protein-1(CKIP-1)is important in the development of osteoblasts and cardiomyocytes.However,the effects of CKIP-1 on osteoblast precursor mesenchymal stem cells(MSCs)remain unclear.This study aimed to determine whether CKIP-1 affects osteogenic differentiation in MSCs and explore the relationship of CKIP-1 and inflammation.Methods:Bone marrow MSCs of CKIP-1 wild type(WT)and knockout(KO)mice were cultivated in vitro.Cell phenotype was analyzed by flow cytometry,colony formation was detected to study the proliferative ability.Osteogenic and adipogenic induction were performed.The osteogenic ability was explored by alizarin red staining,alkaline phosphatase(ALP)staining and ALP activity detection.Quantitative real-time polymerase chain reaction(qRT-PCR)was carried out to determine the mRNA expression levels of osteoblast marker genes.The adipogenic ability was detected by oil red O staining.Content of the bone was analyzed to observe the differences of bone imaging parameters including trabecular bone volume/tissue volume(BV/TV),bone surface area fraction/trabecular BV,trabecular number(Tb.N),and trabecular spacing(Tb.sp).Interleukin(IL)-1b was injected on WT mice of 2 months old and 18 months old,respectively.Difference in CKIP-1 expression was detected by RT-PCR and western blot.The relationship between CKIP-1 and inflammation was explored by RT-PCR and western blot.Results:ALP assays,alizarin red staining,and qRT-PCR showed that MSCs derived from CKIP-1 KO mice exhibited a stronger capability for osteogenesis.Micro-computed tomography detection showed that among 18-month-old mice,CKIP-1 KO mice presented significantly higher bone mass compared withWTmice(P=0.02).No significant difference was observed in 2-month-old mice.In vivo data showed that expression of CKIP-1 was higher in the bone marrow of aging mice than in young mice(4.3-fold increase at themRNA level,P=0.04).Finally,the expression levels of CKIP-1 in bone marrow(3.2-fold increase at themRNA level,P=0.03)and cul

关 键 词：Bone mesenchymal stem cell Casein kinase 2-interacting protein-1 INTERLEUKIN-1B OSTEOGENESIS 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]