miR-630在喉鳞状细胞癌发展中的调控机制研究  

Regulatory mechanism of miR-630 in the development of laryngeal squamous cell carcinoma

作　　者：刘涛 王玮[1] 周洁[1] 左晶晶[2] 李芬[2] LIU Tao;WANG Wei;ZHOU Jie;ZUO Jing-jing;LI Fen(Department of Otorhinolaryngology,Xiaogan city Center Hospital,Xiaogan,Hubei 432000;Department of Otorhinolaryngology Head and Neck Surgery,People's Hospital of Wuhan University,Wuhan,Hubei 430000,China)

机构地区：[1]孝感市中心医院耳鼻喉科,湖北孝感432000 [2]武汉大学人民医院耳鼻喉头颈外科,湖北武汉430000

出　　处：《热带医学杂志》2020年第8期1013-1016,F0002,共5页Journal of Tropical Medicine

基　　金：吴阶平医学基金会临床科研专项(320.6750.17066)。

摘　　要：目的探讨miR-630在喉鳞状细胞癌(LSCC)发展中的调控机制。方法从武汉科技大学附属孝感市中心医院耳鼻喉科收集获得24例临床LSCC标本和24例癌旁组织。体外培养正常人鼻咽细胞系hacat和人喉癌细胞系TU212,荧光定量PCR测定miR-630和下游靶标TP53INP2的表达情况。miR-630和下游靶标TP53INP2的相关性采用Pearson分析。采用特异性干扰miR-630的SiRNA转染TU212细胞,蛋白印迹监测TP53INP2蛋白表达情况。结果与癌旁组织相比,LSCC组织中miR-630表达明显升高,差异有统计学意义(t=8.882,P<0.000 1);TU212细胞中miR-630的表达明显高于hacat细胞,差异有统计学意义(t=8.303,P<0.000 1)。Ⅲ、Ⅳ期癌组织中miR-630表达比Ⅰ、Ⅱ期癌组织明显升高,差异有统计学意义(t=2.336,P=0.029)。根据预测miR-630的靶基因为TP53INP2。TP53INP2在喉癌组织和细胞系中的表达均下调,差异有统计学意义(P<0.05)。在喉癌组织中TP53INP2的表达与miR-630呈负相关,差异有统计学意义(P<0.05)。在TU212细胞中转染SiRNA后,相对于对照组和NC组,miR-630的表达明显降低,TP53INP2的表达明显升高,差异有统计学意义(P<0.05)。结论 miR-630在LSCC中过表达,与不良预后相关,通过负性调控TP53INP2从而促进肿瘤生长和转移。Objective To investigate the regulation mechanism of miR-630 in the development of laryngeal squamous cell carcinoma(LSCC).Methods 24 clinical LSCC specimens and 24 normal specimens were collected from Xiaogan City Center Hospital.Normal human nasopharyngeal cell line hacat and human laryngeal carcinoma cell line TU212 were cultured in vitro.The expression of miR-630 and downstream target TP53INP2 "was determined by real-time PCR.The correlation between miR-630 and the downstream target TP53INP2 was analyzed by Pearson.TU212 cells were transfected with SiRNA which specifically interfered with miR-630,and the expression of TP53INP2 protein was monitored by Western blot.Results Compared with normal tissues,the expression of miR-630 was significantly increased in LSCC tissues(t=8.882,P<0.000 1).The expression of miR-630 in TU212 cells-was significantly higher than that in hacat cells(t=8.303,P<0.000 1).The expression levels of miR-630 in stage Ⅲ and Ⅳ cancer tissues was significantly higher than those in stageⅠ and Ⅱ(t=2.336.P=0.029).The expression of TP53INP2 was down-regulated in laryngeal carcinoma tissues and cell lines(P<0.05),and the result coincided with the prediction that miR-630 targeting TP53INP2.The expression of TP53INP2 was negatively correlated with miR-630 in laryngeal carcinoma(P<0.05).After transfection of SiRNA in TU212 cells,the expression of miR-630 was significantly decreased compared with the control group and NC group,and the expression of TP53INP2 was significantly increased(P<0.05).Conclusion miR-630 was overexpressed in LSCC and was associated with poor prognosis.It might promote tumor growth and metastasis by negatively regulating TP53INP2.

关 键 词：喉鳞状细胞癌 病理分期 淋巴转移 miR-630 TP53INP2 

分 类 号：R739.65[医药卫生—肿瘤]